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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

HYPERTONIC SALINE SOLUTION REDUCES MICROCIRCULATORY DYSFUNCTION AND INFLAMMATION IN A RAT MODEL OF BRAIN DEATH

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Author(s):
Correia, Cristiano de Jesus [1] ; Armstrong, Jr., Roberto [1] ; de Carvalho, Priscila Oliveira [1] ; Simas, Rafael [1] ; Janolli Sanchez, Daniela Crisina [1] ; Breithaupt-Faloppa, Ana Cristina [1] ; Sannomiya, Paulina [1] ; Pinho Moreira, Luiz Felipe [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Inst Coracao Incor, Lab Cirurg Pesquisa Cardiovasc, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Shock; v. 51, n. 4, p. 495-501, APR 2019.
Web of Science Citations: 2
Abstract

Background: Brain death (BD) induces hemodynamic instability with microcirculatory hypoperfusion, leading to increased organ inflammation and dysfunction. This study investigated the effects of 7.5% hypertonic saline solution (HSS) on mesenteric microcirculatory dysfunction and inflammation in a rat model of BD. Methods: Male Wistar rats were anesthetized and mechanically ventilated. BD was induced by rapidly inflating an intracranial balloon catheter. The rats were randomly divided into: SH, sham-operated rats subjected to trepanation; NS, rats treated with NaCl 0.9%, 4 mL/kg immediately after BD; T-1, rats treated with HSS (NaCl 7.5%, 4 mL/kg) immediately or 60 min after BD, T-60. All groups were analyzed 180 min after the start of the experiment. Results: Rats in BD groups presented with a similar hypertensive peak, followed by hypotension. Proportion of perfused small vessels was decreased in the NS group (46%) compared with the SH group (74%, P = 0.0039). HSS restored the proportion of perfused vessels (T-1 = 71%, P = 0.0018). The anti-endothelial nitric oxide synthase (eNOS) protein expression significantly increased in rats given HSS (T-1, and T-60, P = 0.0002). Similar results were observed regarding endothelin-1 (P<0.0001). Increased numbers of rolling (P = 0.0015) and migrated (P = 0.0063) leukocytes were observed in the NS group compared with the SH group. Rats given HSS demonstrated an overall reduction in leukocyte-endothelial interactions. The ICAM-1 levels increased in the NS group compared with the SH group, and decreased in the HSS-treated groups (P = 0.0002). Conclusions: HSS may improve the density of mesenteric perfused small vessels due to its effects on eNOS and endothelin-1 protein expression, and reduces inflammation by decreasing leukocyte adhesion and migration in a rat model of BD. (AU)

FAPESP's process: 12/19841-2 - Study of the effects of hypertonic saline solution on microcirculatory and cardiac tissue changes, and development of the inflammatory process in a rat model of brain death
Grantee:Luiz Felipe Pinho Moreira
Support type: Regular Research Grants