| Full text | |
| Author(s): |
Correia, Cristiano de Jesus
[1]
;
Armstrong, Jr., Roberto
[1]
;
de Carvalho, Priscila Oliveira
[1]
;
Simas, Rafael
[1]
;
Janolli Sanchez, Daniela Crisina
[1]
;
Breithaupt-Faloppa, Ana Cristina
[1]
;
Sannomiya, Paulina
[1]
;
Pinho Moreira, Luiz Felipe
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med, Hosp Clin HCFMUSP, Inst Coracao Incor, Lab Cirurg Pesquisa Cardiovasc, Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Shock; v. 51, n. 4, p. 495-501, APR 2019. |
| Web of Science Citations: | 2 |
| Abstract | |
Background: Brain death (BD) induces hemodynamic instability with microcirculatory hypoperfusion, leading to increased organ inflammation and dysfunction. This study investigated the effects of 7.5% hypertonic saline solution (HSS) on mesenteric microcirculatory dysfunction and inflammation in a rat model of BD. Methods: Male Wistar rats were anesthetized and mechanically ventilated. BD was induced by rapidly inflating an intracranial balloon catheter. The rats were randomly divided into: SH, sham-operated rats subjected to trepanation; NS, rats treated with NaCl 0.9%, 4 mL/kg immediately after BD; T-1, rats treated with HSS (NaCl 7.5%, 4 mL/kg) immediately or 60 min after BD, T-60. All groups were analyzed 180 min after the start of the experiment. Results: Rats in BD groups presented with a similar hypertensive peak, followed by hypotension. Proportion of perfused small vessels was decreased in the NS group (46%) compared with the SH group (74%, P = 0.0039). HSS restored the proportion of perfused vessels (T-1 = 71%, P = 0.0018). The anti-endothelial nitric oxide synthase (eNOS) protein expression significantly increased in rats given HSS (T-1, and T-60, P = 0.0002). Similar results were observed regarding endothelin-1 (P<0.0001). Increased numbers of rolling (P = 0.0015) and migrated (P = 0.0063) leukocytes were observed in the NS group compared with the SH group. Rats given HSS demonstrated an overall reduction in leukocyte-endothelial interactions. The ICAM-1 levels increased in the NS group compared with the SH group, and decreased in the HSS-treated groups (P = 0.0002). Conclusions: HSS may improve the density of mesenteric perfused small vessels due to its effects on eNOS and endothelin-1 protein expression, and reduces inflammation by decreasing leukocyte adhesion and migration in a rat model of BD. (AU) | |
| FAPESP's process: | 12/19841-2 - Study of the effects of hypertonic saline solution on microcirculatory and cardiac tissue changes, and development of the inflammatory process in a rat model of brain death |
| Grantee: | Luiz Felipe Pinho Moreira |
| Support type: | Regular Research Grants |