Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera

Autor(es)

Menezes, Rodolpho S. T. ^{[1, 2]} ; Gazoni, Thiago ^[3] ; Costa, Marco A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Santa Cruz, Dept Ciencias Biol, Rodovia Jorge Amado, Km 16, BR-45662900 Ilheus, BA - Brazil

^[2] Univ Sao Paulo FFCLRP USP, Fac Filosofia Ciencias & Letras, Dept Biol, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Biol, Ave 24A, 1515, BR-13506000 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biological Journal of the Linnean Society; v. 126, n. 4, p. 925-935, APR 2019.
• Citações Web of Science: 0

Resumo

Chromosomal regions enriched in repetitive DNA sequences have a predisposition to instability, resulting in a propensity to undergo structural rearrangements. Hence, the rRNA multigene family, a type of tandem repeat sequences, is a valuable cytogenetic marker for studying genomic organization and chromosomal differentiation. To investigate whether the evolutionary dynamics of heterochromatin and rDNA clusters played a role in the genomic reorganization and chromosomal evolution of a social wasp genus, we used the C-banding technique and fluorescence in situ hybridization (FISH) to conduct chromosomal mapping of the major rDNA class in three closely related species of warrior wasps: Synoeca cyanea, S. ilheensis and S. surinama. In this study, the S. surinama karyotype is reported for the first time. It contains 2n = 34 regular-sized chromosomes and approximately 30 microchromosomes, thus representing an unprecedented number of this chromosomal type for the order Hymenoptera. We discuss the putative evolutionary mechanism for the origin of microchromosomes in S. surinama. For the three species, C-positive heterochromatic blocks were predominantly detected in the pericentromeric region of all chromosomes. However, our FISH results revealed striking variability in the number and chromosomal position of rDNA clusters among the three species studied, as well as intraspecific variations in S. cyanea and S. ilheensis, which suggests intense evolutionary dynamics of this repetitive DNA family and highlights rapid chromosomal evolution in these insects. (AU)

• Processo FAPESP: 15/02432-0 - Organização e evolução cromossômica de elementos repetitivos em genomas de vespas sociais
• Beneficiário: Rodolpho Santos Telles de Menezes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/21098-7 - Associando evolução cromossômica e diversificação em vespas enxameadoras neotropicais através de uma abordagem filogenômica
• Beneficiário: Rodolpho Santos Telles de Menezes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 11/09477-9 - Taxonomia, filogenia, biogeografia e evolução de abelhas Neopasiphaeinae (Hymenoptera: Colletidae) utilizando dados moleculares e morfológicos
• Beneficiário: Eduardo Andrade Botelho de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores