Integrating Molecular Network and Culture Media Variation to Explore the Production of Bioactive Metabolites by Vibrio diabolicus A1SM3

Autor(es)

Conde-Martinez, Natalia ^[1] ; Bauermeister, Anelize ^{[2, 3]} ; Pilon, Alan Cesar ^[2] ; Lopes, Norberto Peporine ^[2] ; Tello, Edisson ^[1]

Afiliação do(s) autor(es)

^[1] Univ La Sabana, Fac Engn, Biosci Doctoral Program, Grp Invest Bioprospecc, Campus Puente Comun, Km 7, Chia 250001 - Colombia

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Farmacol, Inst Ciencias Biomed, BR-05508900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE DRUGS; v. 17, n. 4 APR 2019.
• Citações Web of Science: 0

Resumo

Vibrio diabolicus A1SM3 strain was isolated from a sediment sample from Manaure Solar Saltern in La Guajira and the produced crude extracts have shown antibacterial activity against methicillin-resistant Staphylococcus aureus and cytotoxic activity against human lung cell line. Thus, the aim of this research was to identify the main compound responsible for the biological activity observed and to systematically study how each carbon and nitrogen source in the growth media, and variation of the salinity, affect its production. For the characterization of the bioactive metabolites, 15 fractions obtained from Vibrio diabolicus A1SM3 crude extract were analyzed by HPLC-MS/MS and their activity was established. The bioactive fractions were dereplicated with Antibase and Marinlit databases, which combined with nuclear magnetic resonance (NMR) spectra and fragmentation by MS/MS, led to the identification of 2,2-di(3-indolyl)-3-indolone (isotrisindoline), an indole-derivative antibiotic, previously isolated from marine organisms. The influence of the variations of the culture media in isotrisindoline production was established by molecular network and MZmine showing that the media containing starch and peptone at 7% NaCl was the best culture media to produce it. Also, polyhydroxybutyrates (PHB) identification was established by MS/MS mainly in casamino acids media, contributing to the first report on PHB production by this strain. (AU)

• Processo FAPESP: 16/13292-8 - Análise do conteúdo nitrogenado em áreas sob ação atrófica na Floresta Amazônica e Atlântica utilizando de ferramentas metabolômicas
• Beneficiário: Alan Cesar Pilon
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/17648-4 - Abordagem integrada na prospecção sustentável de produtos naturais marinhos: da diversidade a substâncias anticâncer
• Beneficiário: Anelize Bauermeister
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado