Antibiotic Korormicin A Kills Bacteria by Producing Reactive Oxygen Species

Autor(es)

Maynard, Adam ^{[1, 2, 3]} ; Butler, Nicole L. ^{[4, 1]} ; Ito, Takeshi ^[1] ; da Silva, Adilson Jose ^{[1, 5, 6]} ; Murai, Masatoshi ^[7] ; Chen, Tsute ^{[8, 9]} ; Koffas, Mattheos A. G. ^{[1, 5, 2]} ; Miyoshi, Hideto ^[7] ; Barquera, Blanca ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 - USA

^[2] Rensselaer Polytech Inst, Dept Biol Sci, Troy, NY 12180 - USA

^[3] Harvard Med Sch, Biol & Biomed Sci Phd Program, Boston, MA 02115 - USA

^[4] Rensselaer Polytech Inst, Dept Chem & Chem Biol, Troy, NY - USA

^[5] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Troy, NY - USA

^[6] Univ Fed Sao Carlos, Chem Engn Dept, Sao Paulo - Brazil

^[7] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Kyoto - Japan

^[8] Forsyth Inst, Cambridge, MA - USA

^[9] Harvard Univ, Sch Dent Med, 188 Longwood Ave, Boston, MA 02115 - USA

• Tipo de documento: Artigo Científico
• Fonte: Journal of Bacteriology; v. 201, n. 11 JUN 2019.
• Citações Web of Science: 1

Resumo

Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Instead, perturbation of electron transfer inside the enzyme promotes a reaction between O-2 and one or more redox cofactors in the enzyme (likely the flavin adenine dinucleotide {[}FAD] and 2Fe-2S center), leading to the production of reactive oxygen species (ROS). All Pseudoalteromonas contain the my operon in their genomes, including Pseudoalteromonas strain J010, which produces korormicin. We present activity data indicating that this strain expresses an active Na+-NQR and that this enzyme is not susceptible to korormicin inhibition. On the basis of our DNA sequence data, we show that the Na+-NQR of Pseudoalteromonas J010 carries an amino acid substitution (NqrB-G141A; Vibrio cholerae numbering) that in other Na+-NQRs confers resistance against korormicin. This is likely the reason that a functional Na+-NQR is able to exist in a bacterium that produces a compound that typically inhibits this enzyme and causes cell death. Korormicin is an effective antibiotic against such pathogens as Vibrio cholerae, Ahivibrio fischeri, and Pseudomonas aeruginosa but has no effect on Bacteroides fragilis and Bacteroides thetaiotaomicron, microorganisms that are important members of the human intestinal microflora. IMPORTANCE As multidrug antibiotic resistance in pathogenic bacteria continues to rise, there is a critical need for novel antimicrobial agents. An essential requirement for a useful antibiotic is that it selectively targets bacteria without significant effects on the eukaryotic hosts. Korormicin is an excellent candidate in this respect because it targets a unique respiratory enzyme found only in prokaryotes, the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR). Korormicin is synthesized by some species of the marine bacterium Pseudoalteromonas and is a potent and specific inhibitor of Na+-NQR, an enzyme that is essential for the survival and proliferation of many Gram-negative human pathogens, including Vibrio cholerae and Pseudomonas aeruginosa, among others. Here, we identified how korormicin selectively kills these bacteria. The binding of korormicin to Na+-NQR promotes the formation of reactive oxygen species generated by the reaction of the FAD and the 2Fe-2S center cofactors with O-2. (AU)

• Processo FAPESP: 17/09695-2 - Elucidação da via de biossíntese do antibiótico korormicina em Pseudoalteromonas J010 e produção heteróloga em E. coli
• Beneficiário: Adilson José da Silva
• Modalidade de apoio: Bolsas no Exterior - Pesquisa