The pre-hatching bovine embryo transforms the uterine luminal metabolite composition in vivo

Autor(es)

Sponchiado, Mariana ^{[1, 2]} ; Gonella-Diaza, Angela M. ^{[1, 3]} ; Rocha, Cecilia C. ^[1] ; Lo Turco, Edson G. ^[4] ; Pugliesi, Guilherme ^[1] ; Leroy, Jo L. M. R. ^[2] ; Binelli, Mario ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Pirassununga, SP - Brazil

^[2] Univ Antwerp, Fac Biomed Pharmaceut & Vet Sci, Gamete Res Ctr, Antwerp - Belgium

^[3] Univ Florida, Inst Food & Agr Sci, North Florida Res & Educ Ctr, Marianna, FL - USA

^[4] Sao Paulo Fed Univ, Dept Surg, Human Reprod Sect, Div Urol, Sao Paulo, SP - Brazil

^[5] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, JUN 7 2019.
• Citações Web of Science: 1

Resumo

In cattle, conceptus development after elongation relies on well-characterized, paracrine interactions with the hosting maternal reproductive tract. However, it was unrecognized previously that the pre-hatching, pre-implantation bovine embryo also engages in biochemical signalling with the maternal uterus. Our recent work showed that the embryo modified the endometrial transcriptome in vivo. Here, we hypothesized that the embryo modulates the biochemical composition of the uterine luminal fluid (ULF) in the most cranial portion of the uterine horn ipsilateral to the corpus luteum. Endometrial samples and ULF were collected post-mortem from sham-inseminated cows and from cows inseminated and detected pregnant 7 days after oestrus. We used quantitative mass spectrometry to demonstrate that the pre-hatching embryo changes ULF composition in vivo. Embryo-induced modulation included an increase in concentrations of lipoxygenase-derived metabolites {[}12(S)-HETE, 15(S)-HETE] and a decrease in the concentrations of amino acids (glycine), biogenic amines (sarcosine), acylcarnitines and phospholipids. The changed composition of the ULF could be due to secretion or depletion of specific molecules, executed by either the embryo or the endometrium, but initiated by signals coming from the embryo. This study provides the basis for further understanding embryo-initiated modulation of the uterine milieu. Early embryonic signalling may be necessary to guarantee optimal development and successful establishment of pregnancy in cattle. (AU)

• Processo FAPESP: 11/03226-4 - Assinaturas da receptividade
• Beneficiário: Mario Binelli
• Modalidade de apoio: Auxílio à Pesquisa - Temático