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Proenca-Modena, Jose Luiz ^[1] ; Cardoso, Ricardo de Souza ^[2] ; Criado, Miria Ferreira ^{[3, 2]} ; Milanez, Guilherme Paier ^[1] ; de Souza, William Marciel ^[3] ; Parise, Pierina Lorencini ^[1] ; Bertol, Jessica Wildgrube ^[1] ; Santos de Jesus, Bruna Lais ^[2] ; Moreira Prates, Mirela Cristina ^[2] ; Silva, Maria Lucia ^{[3, 2]} ; Buzatto, Guilherme Pietrucci ^[4] ; Demarco, Ricardo Cassiano ^[4] ; Pereira Valera, Fabiana Cardoso ^[4] ; Tamashiro, Edwin ^[4] ; Anselmo-Lima, Wilma Terezinha ^[4] ; Arruda, Eurico ^{[3, 2]}

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Rua Monteiro Lobato 255, Cidade Univ, BR-13083862 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell Biol, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Virol Res Ctr, Dept Cell Biol, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Ophthalmol Otorhinolaryngol & Head & Surg, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medical Virology; v. 91, n. 7, p. 1250-1262, JUL 2019.
• Citações Web of Science: 2

Resumo

The role of human adenovirus (HAdV) infection in different acute diseases, such as febrile exudative tonsillitis, conjunctivitis, and pharyngoconjunctival fever is well established. However, the relationships, if any, of HAdV persistence and reactivation in the development of the chronic adenotonsillar disease is not fully understood. The present paper reports a 3-year cross-sectional hospital-based study aimed at detecting and quantifying HAdV DNA and mRNA of the HAdV hexon gene in adenoid and palatine tonsil tissues and nasopharyngeal secretions (NPS) from patients with adenotonsillar hypertrophy or recurrent adenotonsillitis. HAdV C, B, and E were detectable in nearly 50% of the patients, with no association with the severity of airway obstruction, nor with the presence of recurrent tonsillitis, sleep apnea or otitis media with effusion (OME). Despite the higher rates of respiratory viral coinfections in patients with HAdV, the presence of other viruses, including DNA and RNA viruses, had no association with HAdV replication or shedding in secretions. Higher HAdV loads in adenoids showed a significant positive correlation with the presence of sleep apnea and the absence of OME. Although this study indicates that a significant proportion (similar to 85%) of individuals with chronic adenotonsillar diseases have persistent nonproductive HAdV infection, including those by HAdV C, B, and E, epithelial and subepithelial cells in tonsils seem to be critical for HAdV C production and shedding in NPS in some patients, since viral antigen was detected in these regions by immunohistochemistry in four patients, all of which were also positive for HAdV mRNA detection. (AU)

• Processo FAPESP: 16/00194-8 - Patogênese e neurovirulência de vírus emergentes no Brasil
• Beneficiário: José Luiz Proença Módena
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 09/51818-8 - O perfil das infeccoes virais em patologias cronicas do trato respiratorio superior: hipertrofia adenoamigdaliana, otite media secretora e rinossinusite
• Beneficiário: Wilma Terezinha Anselmo Lima
• Modalidade de apoio: Auxílio à Pesquisa - Temático