| Texto completo | |
| Autor(es): |
Goncalves, Leticia C. P.
[1]
;
Mansouri, Hamid R.
[1]
;
PourMehdi, Shads
[1]
;
Abdellah, Mohamed
[2, 3]
;
Fadiga, Bruna S.
[4, 2]
;
Bastos, Erick L.
[4]
;
Sa, Jacinto
[2, 5]
;
Mihovilovic, Marko D.
[1]
;
Rudroffe, Florian
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] TU Wien, Inst Appl Synthet Chem, Getreidemarkt 9 163, A-1060 Vienna - Austria
[2] Uppsala Univ, Angstrom Lab, Dept Chem, Phys Chem Div, S-75120 Uppsala - Sweden
[3] South Valley Univ, Qena Fac Sci, Dept Chem, Qena 83523 - Egypt
[4] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, BR-03178200 Sao Paulo - Brazil
[5] Polish Acad Sci, Inst Phys Chem, PL-01224 Warsaw - Poland
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CATALYSIS SCIENCE & TECHNOLOGY; v. 9, n. 10, p. 2682-2688, MAY 21 2019. |
| Citações Web of Science: | 2 |
| Resumo | |
Light-driven reduction of flavins, e.g. FAD or FMN, by sacrificial electron donors emerged as a convenient method to promote biocatalytic transformations. However, flavin activation has been restricted to oxygen-free conditions to prevent enzyme deactivation caused by reactive oxygen species (ROS). Herein, we show that the photoreduction of FMN by morpholines, including 3-(N-morpholino)propanesulfonic acid (MOPS), lessens the deactivation of the enoate reductase XenB from Pseudomonas sp. during the stereoselective asymmetric enzymatic reduction of a model ,-unsaturated diketone under aerobic conditions, leading to a 91% GC-yield and a stereoselectivity greater than 94%. The kinetic stability of the thermolabile XenB was increased by more than 20-fold in MOPS buffer compared to that in Tris-HCl buffer, and a pronounced positive effect on the transition midpoint temperature was observed. The reactive form of the FMN photocatalyst is stabilized by the formation of a (3){[}FMN--MOPS+] ensemble, which reduces the formation of hydrogen peroxide and other ROS in the presence of oxygen. These results contribute to broaden the application of photobiocatalytic transformations using flavin-dependent reductases. (AU) | |
| Processo FAPESP: | 17/50130-9 - Bioinspired photoactive electron donors. (sprint 1/2017) |
| Beneficiário: | Erick Leite Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |