Isoflavones improve collagen I and glycosaminoglycans and prevent bone loss in type 1 diabetic rats

Autor(es):Mostrar menos -

Carbonel, A. A. F. ^[1] ; Vieira, M. C. ^[2] ; Simoes, R. S. ^[3] ; Lima, P. D. A. ^[4] ; Fuchs, L. F. P. ^[2] ; Girao, E. R. C. ^[2] ; Cicivizzo, G. P. ^[1] ; Sasso, G. R. S. ^[2] ; Carvalho de Moraes, L. O. ^[1] ; Soares Junior, J. M. ^[3] ; Baracat, E. C. ^[3] ; Simoes, M. J. ^[1] ; Girao, M. J. B. C. ^[2]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Morphol & Genet, Rua Botucatu, 740 Edificio Lemos Torres, BR-04023062 Sao Paulo, SP - Brazil

^[2] Fed Univ Sao Paulo EPM UNIFESP, Paulista Sch Med, Dept Gynecol, Sao Paulo - Brazil

^[3] Univ Sao Paulo FMUSP, Med Fac, Dept Obstet & Gynecol, Sao Paulo - Brazil

^[4] Univ Ottawa, Dept Cellular & Mol Med, Ottawa, ON - Canada

• Tipo de documento: Artigo Científico
• Fonte: CLIMACTERIC; v. 23, n. 1 JUN 2019.
• Citações Web of Science: 0

Resumo

Objective: The objective of this study was to evaluate the action of soy isoflavones (ISO) and 17 beta-estradiol on collagen I (CollI) and sulfated glycosaminoglycans (GAGs) in the bone matrix of diabetic rats. Methods: Sixty adult female rats (Rattus norvegicus albinus) underwent ovariectomy, and then were randomized into six groups of 10 animals each: GI, sham control ovariectomized animals; GII, sham control diabetic (DM) ovariectomized animals; GIII, control ovariectomized animals receiving propylene glycol vehicle; GIV, control ovariectomized DM animals receiving propylene glycol vehicle; GV, ovariectomized DM animals treated with ISO (150 mg/kg by gavage); and GVI, ovariectomized DM animals treated with estrogen (17 beta-estradiol, 10 mg/kg, subcutaneously). 17 beta-Estradiol was used as a positive control when compared with ISO. To obtain significant depletion of the estrogen levels and subsequent bone loss, a postsurgical period of 90 days was observed. Treatments occurred during 30 consecutive days. After euthanasia, shafts of the animals' femurs were immersed in liquid nitrogen for molecular biology analysis, and the distal femurs were removed and processed for paraffin embedding. Results: ISO (GV) and 17 beta-estradiol (GVI) improved bone formation, increasing GAGs and CollI formation when compared to the control group (GIV) (p < 0.05). Conclusions: ISO and 17 beta-estradiol contribute to the decrease of bone loss in diabetic rats. (AU)

• Processo FAPESP: 14/17077-9 - Efeitos das isoflavonas ou 17beta-estradiol na interação matriz-célula no endométrio de ratas diabéticas: relevância para a terapia hormonal
• Beneficiário: Adriana Aparecida Carbonel Castro Ferreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado