A NOVEL Scytalidium SPECIES: UNDERSTAND THE CELLULOLYTIC SYSTEM FOR BIOMASS SACCHARIFICATION

Autor(es)

Delabona, Priscila da S. ^{[1, 2]} ; Silva, Mateus R. ^[1] ; Paixao, Douglas A. A. ^[1] ; Lima, Deise J. ^[1] ; Rodrigues, Gisele N. ^[1] ; Lee, Marcela do S. ^[3] ; Souza, Marcia G. da S. ^[3] ; Bussamra, Bianca C. ^[1] ; Santos, Alberdan S. ^[3] ; Pradella, Jose G. da C. ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Ciencia & Tecnol Bioetanol, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Programa Posgrad Genet & Biol Mol, Campinas, SP - Brazil

^[3] Univ Fed Para, Labs Invest Sistemat Biotecnol & Biodiversidade M, Belem, PA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Chemical Engineering; v. 36, n. 1, p. 85-97, JAN-MAR 2019.
• Citações Web of Science: 0

Resumo

ABSTRACT In order to overcome the bottlenecks related to lignocellulosic-derived sugars, the search for more efficient enzymatic cocktails, containing a broad-spectrum of specific activities, relies on an important feature. This paper describes new enzyme activities derived from the novel strain of the Scytalidium genus isolated from the Amazonas rainforest. The production of the enzymatic cocktail was induced by delignified-hydrothermal bagasse (DHB), and yeast extract was used to improve secretion activities, resulting in a positive influence on total cellulase activity. The enzymatic cocktail produced by this novel strain contains specific activities for biomass degradation, including FPAse, xylanase and β-glucosidase. Moreover, it is capable of hydrolyzing 62% of the alkaline pretreated bagasse, surpassing in 14% the hydrolytic capability achieved by the commercial cocktail Celluclast. To this extent, the strain described here emerges as a reliable alternative to other available enzymes and, consequently, amplification of available specific substrate activities. (AU)

• Processo FAPESP: 10/51309-3 - Biodiversidade para produção de etanol de segunda geração
• Beneficiário: Carlos Eduardo Vaz Rossell
• Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE