Co-Expression Networks Reveal Potential Regulatory Roles of miRNAs in Fatty Acid Composition of Nelore Cattle

Autor(es):Mostrar menos -

de Oliveira, Priscila S. N. ^[1] ; Coutinho, Luiz L. ^[2] ; Cesar, Aline S. M. ^[3] ; da Silva Diniz, Wellison J. ^[4] ; de Souza, Marcela M. ^[5] ; Andrade, Bruno G. ^[1] ; Koltes, James E. ^[5] ; Mourao, Gerson B. ^[3] ; Zerlotini, Adhemar ^[6] ; Reecy, James M. ^[5] ; Regitano, Luciana C. A. ^[1]

Afiliação do(s) autor(es)

^[1] Empresa Brasileira Pesquisa Agr, Embrapa Pecuaria Sudeste, Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Dept Anim Sci, Piracicaba - Brazil

^[3] Univ Sao Paulo, Dept Agroind Food & Nutr, Piracicaba - Brazil

^[4] Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP - Brazil

^[5] Iowa State Univ, Dept Anim Sci, Ames, IA - USA

^[6] Embrapa Informat Agr, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN GENETICS; v. 10, JUL 11 2019.
• Citações Web of Science: 1

Resumo

Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were obtained from skeletal muscle of Nelore cattle that had extreme genomic breeding values for OA and CLA. Insulin and MAPK signaling pathways were identified by WGCNA as central pathways associated with both of these fatty acids. Co-expression network analysis identified bta-miR-33a/b, bta-miR-100, bta-miR-204, bta-miR-365-5p, bta-miR-660, bta-miR-411a, bta-miR-136, bta-miR-30-5p, bta-miR-146b, bta-let-7a-5p, bta-let-7f, bta-let-7, bta-miR 339, bta-miR-10b, bta-miR 486, and the genes ACTA1 and ALDOA as potential regulators of fatty acid synthesis. This study provides evidence and insights into the molecular mechanisms and potential target genes involved in fatty acid content differences in Nelore beef cattle, revealing new candidate pathways of phenotype modulation that could positively benefit beef production and human consumption. (AU)

• Processo FAPESP: 12/23638-8 - Bases moleculares da qualidade da carne em bovinos da raça Nelore
• Beneficiário: Luciana Correia de Almeida Regitano
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/03291-4 - Perfil de expressão entre espécies de miRNAs relacionados à ácidos graxos
• Beneficiário: Priscila Silva Neubern de Oliveira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado