Early diagnosis of Zika infection using a ZnO nanostructures-based rapid electrochemical biosensor

Autor(es)

Faria, Aline Macedo ^[1] ; Mazon, Talita ^[1]

Afiliação do(s) autor(es)

^[1] CTI, Ctr Tecnol Informacao Renato Archer, Rod D Pedro 1, KM 143-6, BR-13069901 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 203, p. 153-160, OCT 1 2019.
• Citações Web of Science: 0

Resumo

In this work, we report a develop of electrochemical immunosensor based on ZnO nanostructures immobilized with ZIKV-NS1 antibody on Printed Circuit Board (PCB). ZnO nanostructures were grown on PCB by chemical bath deposition (CDB) and characterized by SEM. ZIKV-NS1 antibody was immobilized on the ZnO nanostructures surface via cystamine and glutaraldehyde. The samples were characterized by Immunofluorescence Confocal Microscopy and FTIR to identify the immobilization of the antibody to the sensor board. The analytical responses of the immunosensor were evaluated by Cyclic Voltammetry (CV). The biosensor developed here allows rapid detection of Zika virus in undiluted urine, without cross reactive with DENV-NS1 antigen, with linear range 0.1 ng mL(-1) to 100 ng mL(-1). The limit of detection is lower than 1.00 pg mL(-1). The biosensor is portable, cost-effectiveness, and simple to use, which makes it ideal for point-of-care applications. (AU)

• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs