Could a chelant improve the effect of curcumin-mediated photodynamic antimicrobial chemotherapy against dental intact biofilms?

Autor(es)

Cusicanqui Mendez, Daniela Alejandra ^[1] ; Rizzato, Veridiana Lopes ^[1] ; Chaves Lamarque, Giuliana Campos ^[1] ; Dionisio, Evandro Jose ^[1] ; Rabelo Buzalaf, Marilia Afonso ^[2] ; Rios, Daniela ^[1] ; Andrade Moreira Machado, Maria Aparecida ^[1] ; Cruvinel, Thiago ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Alameda Dr Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 34, n. 6, p. 1185-1192, AUG 2019.
• Citações Web of Science: 0

Resumo

To our knowledge, there is still no evidence in relation to the combination of curcumin with chelants to improve the effects of antimicrobial photodynamic therapy (aPDT) on complex dental caries biofilms. Therefore, the aim of this study was to evaluate the antimicrobial effect of curcumin-ethylenediaminetetraacetic acid (EDTA)-mediated aPDT on the vitality of intact biofilms of dentin caries microcosms. Biofilms were grown on glass slabs in McBain medium plus 1% sucrose in microaerophily at 37 degrees C for 5days. Then, biofilms were treated with associations of 600 mu molL(-1) curcumin combined or not with 1% EDTA and 37.5 or 75Jcm(-2) LED (455nm). The vitality was determined by a confocal laser scanning microscopy (CLSM) after staining biofilms with a mixture of 2.5gL(-1) fluorescein diacetate and 0.25gL(-1) ethidium bromide. Statistical analysis was conducted by Kruskal-Wallis and post hoc Dunn's test (P<0.05). Three treatments were able to reduce the vitality of overall biofilms: curcumin +75Jcm(-2) LED, curcumin-EDTA +37.5Jcm(-2) LED, and curcumin-EDTA +75Jcm(-2) LED. Also, the vitality of inner layers of biofilms was significantly reduced only after the combination of aPDT with EDTA. Therefore, the association of curcumin and EDTA improved the antimicrobial effect of aPDT on dentin caries microcosms, considering the application of lower light densities and deeper layers of biofilms. (AU)

• Processo FAPESP: 14/22238-1 - Efeito da terapia fotodinâmica antimicrobiana sobre microcosmos de lesões de cárie em dentina
• Beneficiário: Eliézer Gutierres
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica