Th1/Th2/Th17/Treg Balance in Apical Periodontitis of Normoglycemic and Diabetic Rats

Autor(es)

Samuel, Renata Oliveira ^{[1, 2]} ; Ervolino, Edilson ^[3] ; de Azevedo Queiroz, India Olinta ^[1] ; Azuma, Mariane Maffei ^{[1, 4]} ; Ferreira, Gabriela Tiago ^[2] ; Angelo Cintra, Luciano Tavares ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Endodont, R Jose Bonifacio 1193, Aracatuba, SP - Brazil

^[2] Univ Uberaba, Sch Dent, Dept Clin Dent, Uberaba, MG - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba, SP - Brazil

^[4] Univ Michigan, Dept Cariol Restorat Sci & Endododont, Ann Arbor, MI 48109 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 45, n. 8, p. 1009-1015, AUG 2019.
• Citações Web of Science: 2

Resumo

Introduction: The aim of this study was to evaluate the inflammatory profile of T helper (Th) cells in normoglycemic (N) and diabetic rats with apical periodontitis (AP). Methods: Twenty male Wistar rats were divided in 2 groups: N rats and rats with diabetes mellitus (DM). DM was induced using streptozotocin, and AP was induced by dental pulp exposure of the first mandibular molar to the oral environment. After 30 days, the mandibles were removed and processed for histologic analysis, bacterial analysis, and immunochemical assays for interleukin (IL)-6, tumor necrosis factor alpha, IL-17, IL-23, interferon gamma, and IL-10. The Mann-Whitney U test and Student t test were used for statistical analysis (P < .05). Results: The DM group showed more intense inflammatory infiltrate with larger sizes of bone reabsorption and a greater presence of bacteria than the N group (P < .05). Proinflammatory cytokine levels in the DM group were also greater than those in the N group (P < .05). However, interferon gamma was more intense in the N group than in the DM group (P < .05). Conclusions: The inflammatory profile of AP in DM is different from that in the N group, suggesting that Th1 is a secondary strain and the Th17 strain is predominant in DM. (AU)

• Processo FAPESP: 13/23358-8 - Perfil inflamatório local e sistêmico de ratos wistar com múltiplas infecções endodônticas
• Beneficiário: Renata Oliveira Samuel
• Modalidade de apoio: Bolsas no Brasil - Doutorado