| Texto completo | |
| Autor(es): |
da Rosa Filho, Roberto R.
[1]
;
Angrimani, Daniel S. R.
[1]
;
Brito, Maira M.
[1]
;
Nichi, Marcilio
[1]
;
Vannucchi, I, Camila
;
Lucio, Cristina F.
[2]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med & Anim Sci, Campus Sao Paulo, Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil
[2] Vannucchi, Camila, I, Univ Sao Paulo, Dept Anim Reprod, Sch Vet Med & Anim Sci, Campus Sao Paulo, Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ANIMAL BIOTECHNOLOGY; v. 32, n. 1 AUG 2019. |
| Citações Web of Science: | 1 |
| Resumo | |
In order to add information to physiology of sperm maturation and help to underline future antioxidant supplementation treatment to epididymal sperm, the aim of this study was to evaluate susceptibility of caput, corpus and cauda epididymal sperm to different reactive oxygen species (ROS) in dogs. Epididymal sperm was separately collected from each segment (caput, corpus and cauda) and subjected to 4 different ROS-challenges: superoxide anion, hydrogen peroxide, hydroxyl radical, malondialdehyde (MDA) or maintained as control. After 30 min of incubation in each ROS, sperm was evaluated for sperm kinetics, plasma and acrossomal membrane integrity, mitochondrial activity and resistance to oxidative stress. Decreased total and progressive sperm motility and rapid velocity at epididymal corpus occurred after exposure to hydrogen peroxide, hydroxyl radical and MDA. However, for cauda epididymis, hydrogen peroxide and malondialdehyde promoted higher deleterious effect regarding sperm motility and velocity. Only at cauda epididymis MDA decreased sperm mitochondrial activity index and no kinetics alterations (motility or velocity) occurred after exposure to superoxide anion in corpus and cauda epididymis. In conclusion, corpus and cauda epididymal sperm are highly susceptible to deleterious effect of hydrogen peroxide, malondialdehyde and hydroxyl radical. In addition, epididymal canine sperm is relatively resistant to superoxide anion damage. (AU) | |
| Processo FAPESP: | 15/00597-2 - Estudo da sensibilidade às espécies reativas de oxigênio durante a maturação espermática em cães |
| Beneficiário: | Roberto Rodrigues da Rosa Filho |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |