Determination of tryptoquialanines A and C produced by Penicillium digitatum in oranges: Are we safe?

Autor(es)

Araujo, Eder de Vilhena ^[1] ; Vendramini, Pedro Henrique ^[1] ; Costa, Jonas Henrique ^[1] ; Eberlin, Marcos Nogueira ^[1] ; Montagner, Cassiana Carolina ^[1] ; Fill, Taicia Pacheco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, CP 6154, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 301, DEC 15 2019.
• Citações Web of Science: 0

Resumo

A method was developed and validated for determination of tryptoquialanines A and C in orange samples on epicarp (exterior peel), mesocarp (white peel), and endocarp (fruit juice) based on QuEChERS extraction and LC-MS/MS analysis. The method showed an excellent linearity over a range of 5-400 mu g kg(-1), with r(2) >= 0.998. The limits of detection (LOD) and quantification (LOQ) were 5 and 10 mu g kg(-1), respectively. Recoveries showed values between 57 and 101%, with RSD <= 12%. Analysis of infected oranges showed diffusion of the alkaloids between the orange layers after 4 days post infection in concentrations > LOQ. Mycotoxin diffusion to healthy oranges after direct contact with infected oranges for 48 h, showed alkaloid concentrations >= 10 mu g kg(-1) on epicarp layer. The developed method can be easily applied for quality control in routine analysis of orange fruit due to the high risk that these tremorgenic alkaloids represent to human health. (AU)

• Processo FAPESP: 18/03670-0 - Prospecção de metabólitos secundários com atividade anticâncer produzidos por fungos fitopatogênicos
• Beneficiário: Jaqueline Moraes Bazioli
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/10384-6 - Estudos biossintéticos utilizando abordagens bottom-up de policetídeos produzidos por fungos do gênero Penicillium
• Beneficiário: Taicia Pacheco Fill
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/24462-4 - Entendendo as estratégias de virulência do fungo Penicillium digitam contra seu hospedeiro citros
• Beneficiário: Taicia Pacheco Fill
• Modalidade de apoio: Auxílio à Pesquisa - Regular