Occurrence and genetic diversity of hemoplasmas in beef cattle from the Brazilian Pantanal, an endemic area for bovine trypanosomiasis in South America

Autor(es)

Califre de Mello, Victoria Valente ^{[1, 2]} ; de Souza Ramos, Inalda Angelica ^[1] ; Herrera, Heitor Miraglia ^[3] ; Mendes, Natalia Serra ^{[1, 2]} ; Calchi, Ana Claudia ^[1] ; Vilela Campos, Joao Bosco ^[3] ; Macedo, Gabriel Carvalho ^[3] ; Almeida Alves, Joao Vitor ^[3] ; Machado, Rosangela Zacarias ^[1] ; Andre, Marcos Rogerio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, FCAV, UNESP, Lab Immunoparasitol, Dept Vet Pathol, Jaboticabal, SP - Brazil

^[2] Univ Estadual Paulista, FCAV, UNESP, Grad Program Agr Microbiol, Jaboticabal, SP - Brazil

^[3] Univ Catolica Dom Bosco, Campo Grande, Mato Grosso Do - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES; v. 66, OCT 2019.
• Citações Web of Science: 0

Resumo

Hemotropic mycoplasmas (hemoplasmas) are Gram-negative bacteria that parasitize the erythrocyte surface of a wide variety of mammals. The present study aimed at investigating the occurrence of hemoplasmas in beef cattle in the Brazilian Pantanal, an area endemic for bovine trypanosomiasis in South America. Additionally, the objective of this study was to characterize molecularly the genotypes of the found hemoplasmas. For this purpose, blood and serum samples of 400 beef cattle were collected from five properties in Corumba, Nhecolandia sub-region, Mato Grosso do Sul, in Midwest Brazil. Blood samples underwent DNA extraction and standard 16S rRNA gene-based PCR assays for hemoplasmas. The sequences obtained were submitted to phylogenetic inferences, distance analysis, and genotype diversity. The Indirect Enzyme-Linked Immunoabsorbent Assay (iELISA) indicated the presence of anti-Trypanosoma vivax IgG antibodies in 89.75% of the animals sampled, confirming the endemicity of said agent in the studied region. Among the 400 bovine blood samples tested, 2.25% (9/400) were positive for hemoplasmas in cPCR. The phylogenetic analysis of the obtained sequences confirmed the presence of `Candidatus Mycoplasma haemobos' and Mycoplasma wenyonii DNA in 0.5% (2/400) and 1.75% (7/400) animals, respectively. Five genotypes of M. wenyonii and one of `Candidatus M. haemobos' were detected among the sequenced amplicons. The present study showed low molecular occurrence of haemoplasmas in beef cattle sampled in the Brazilian Pantanal, an area endemic for bovine trypanosomiasis. Despite of the conservation of the 16S rRNA gene, there was considerable diversity of hemoplasma genotypes infecting the sampled beef cattle. (AU)

• Processo FAPESP: 15/14896-1 - Detecção e caracterização molecular de agentes das famílias Anaplasmataceae e Bartonellaceae em animais selvagens e ectoparasitas no Brasil
• Beneficiário: Marcos Rogério André
• Modalidade de apoio: Auxílio à Pesquisa - Regular