Gloeosporiocide, a new antifungal cyclic peptide from Streptomyces morookaense AM25 isolated from the Amazon bulk soil

Autor(es)

dos Reis, Gislaine Vicente ^[1] ; Abraham, Wolf-Rainer ^[2] ; Grigoletto, Diana Fortkamp ^{[1, 2]} ; de Campos, Jessica Bueno ^[3] ; Marcon, Joelma ^[3] ; da Silva, Jose Antonio ^[3] ; Quecine, Maria Carolina ^[3] ; de Azevedo, Joao Lucio ^[3] ; Ferreira, Antonio Gilberto ^[4] ; de Lira, Simone Possedente ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Exact Sci, Coll Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[2] Helmholtz Ctr Infect Res, Chem Microbiol, D-38124 Braunschweig - Germany

^[3] Univ Sao Paulo, Dept Genet, Coll Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEMS Microbiology Letters; v. 366, n. 14 JUL 2019.
• Citações Web of Science: 0

Resumo

Actinobacteria are known by their ability to produce several antimicrobial compounds of biotechnological interest. Thus, in this study, we isolated and identified by partial 16S RNA sequencing similar to 100 actinobacteria isolates from guarana (Paullinia cupana) bulk soil. Besides, we isolated from the actinobacteria Streptomyces morookaense AM25 a novel cyclic peptide, named gloeosporiocide, molecular formula C44H48N11O7S3 (calculated 938.2901), and characterized by the presence of cyclized cysteins to form three thiazols. The novel compound had activity against the plant pathogen Colletotrichum gloeosporioides, assayed by the paper disk diffusion method (42.7% inhibition, 0.1 mg disk(-1)) and by the microdilution assay (1.25 g L-1). Our results reveal the potential of the actinobacteria from the Amazon rhizospheric soils as biocontrol agents as well as producers of new compounds with antifungal activity. Thus, this work constitutes a step forward in the development of the biotechnology of actinobacteria in the production of compounds of agronomic interest. (AU)

• Processo FAPESP: 13/50228-8 - Componentes da biodiversidade, e seus caracteres metabólicos, de ilhas do Brasil: uma abordagem integrada
• Beneficiário: Roberto Gomes de Souza Berlinck
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 14/15760-3 - Potencial biotecnológico de metabólitos secundários de plantas e fungos endofíticos
• Beneficiário: Simone Possedente de Lira
• Modalidade de apoio: Auxílio à Pesquisa - Regular