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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Chromosome spreading of the (TTAGGG)n repeats in the Pipa carvalhoi Miranda-Ribeiro, 1937 (Pipidae, Anura) karyotype

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Autor(es):
Zattera, Michelle Louise [1] ; Lima, Luana [2] ; Duarte, Iraine [1] ; de Sousa, Deborah Yasmin [1] ; dos Santos Araujo, Olivia Gabriela [3] ; Gazoni, Thiago [3] ; Mott, Tami [2] ; Recco-Pimentel, Shirlei Maria [4] ; Bruschi, Daniel Pacheco [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Fed Parana UFPR, Dept Genet, Programa Posgrad Genet, Jardim Amer, Ctr Politecn, BR-81531990 Curitiba, Parana - Brazil
[2] Univ Fed Alagoas UFAL, Inst Ciencias Biol & Saude, Ave Louriva Melo Mota S-N, BR-57072900 Maceio, Alagoas State - Brazil
[3] Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Biol, Campus Rio Claro, Ave 24A 1515, BR-13506900 Rio Claro, SP - Brazil
[4] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Estrutural & Func, Ave Bertrand Russel S-N, BR-13083865 Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: COMPARATIVE CYTOGENETICS; v. 13, n. 3, p. 297-309, OCT 14 2019.
Citações Web of Science: 0
Resumo

Pipidae is a clade of Anura that diverged relatively early from other frogs in the phylogeny of the group. Pipids have a unique combination of morphological features, some of which appear to represent a mix of adaptations to aquatic life and plesiomorphic characters of Anura. The present study describes the karyotype of Pipa carvalhoi Miranda-Ribeiro, 1937, including morphology, heterochromatin distribution, and location of the NOR site. The diploid number of P. carvalhoi is 2n=20, including three metacentric pairs (1, 4, 8), two submetacentric (2 and 7), three subtelocentric (3, 5, 6), and two telocentric pairs (9 and 10). C-banding detected centromeric blocks of heterochromatin in all chromosome pairs and the NOR detected in chromosome pair 9, as confirmed by FISH using the rDNA 28S probe. The telomeric probes indicated the presence of interstitial telomeric sequences (ITSs), primarily in the centromeric region of the chromosomes, frequently associated with heterochromatin, suggesting that these repeats are a significant component of this region. The findings of the present study provide important insights for the understanding of the mechanisms of chromosomal evolution in the genus Pipa, and the diversification of the Pipidae as a whole. (AU)

Processo FAPESP: 16/07717-6 - Marcadores clássicos e moleculares no estudo cromossômico de anuros
Beneficiário:Shirlei Maria Recco-Pimentel
Modalidade de apoio: Auxílio à Pesquisa - Regular