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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Physiological and functional aspects of metal -binding protein associated with mercury in the liver tissue of pirarucu (Arapaima gigas) from the Brazilian Amazon

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Autor(es):
Bataglioli, Izabela da Cunha [1] ; Souza Vieira, Jose Cavalcante [2, 3] ; de Queiroz, Joao Vitor [1] ; Fernandes, Mileni da Silva [4] ; Bittarello, Alis Correia [1] ; Braga, Camila Pereira [5] ; Rabelo Buzalaf, Marilia Afonso [4] ; Adamec, Jiri [5] ; Zara, Luiz Fabricio [6] ; Padilha, Pedro de Magalhaes [2, 1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil
[3] Univ Fed Mato Grosso do Sul, Inst Chem Inqui, Campo Grande - Brazil
[4] Univ Sao Paulo, Bauru - Brazil
[5] Univ Nebraska UNL, Lincoln, NE - USA
[6] Univ Brasilia UnB, Coll Planaltina, Brasilia, DF - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: Chemosphere; v. 236, DEC 2019.
Citações Web of Science: 1
Resumo

High concentrations of mercury found in soils, sediments, fish, and humans of the Amazon region have gained prominence in scientific studies during the last decade. However, studies related to the elucidation of mercury toxicity mechanisms in ichthyofauna at the molecular and metallomic levels that seek to elucidate physiological and functional aspects, as well as the search for biomarkers of mercury exposure, are still sparse. In the search for these answers, the present study analyzed the hepatic tissue proteome of the Arapaima gigas (pirarucu) fish species collected in the Jirau hydroelectric power plant reservoir in the state of Rondonia state, Brazil, in order to identify mercury-related metal-binding proteins and to elucidate their physiological and functional aspects. The proteomic profile of the hepatic tissue of Arapaima gigas was obtained by two-dimensional electrophoresis (2D-PAGE) and the presence of mercury was mapped in the protein SPOTS by graphite furnace atomic absorption spectrometry(GFAAS). Mercury was detected in 18 protein SPOTS with concentrations ranging from 0.13 +/- 0.003 to 131.00 +/- 3 mg kg(-1). The characterization of the protein SPOTS associated with mercury was performed by electrospray ionisation tandem mass spectrometry (ESI-MS/MS), and 10 proteins were identified. Bioinformatics analyses showed that most of the proteins found linked to mercury were involved in cellular component processes and biological processes. For the most part, protein sequences have cellular functions comprising catalytic, binding, sense of localization, and metabolic processes. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 17/09466-3 - Elucidação de aspectos fisiológicos e funcionais de proteínas associadas ao mercúrio em pirarucu (Arapaima gigas)
Beneficiário:Izabela da Cunha Bataglioli
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 16/19404-2 - Caracterização de biomarcadores de toxicidade de mercúrio em peixes da Bacia do Rio Madeira-Rondônia utilizando-se estratégias metaloproteômicas
Beneficiário:Pedro de Magalhães Padilha
Linha de fomento: Auxílio à Pesquisa - Regular