Exposure to an Environmentally Relevant Phthalate Mixture During Prostate Development Induces MicroRNA Upregulation and Transcriptome Modulation in Rats

Autor(es)

Scarano, Wellerson R. ^{[1, 2, 3]} ; Bedrat, Amina ^{[2, 3]} ; Alonso-Costa, Luiz G. ^[1] ; Aquino, Ariana M. ^[1] ; Fantinatti, Bruno E. A. ^[1] ; Justulin, Luis A. ^[1] ; Barbisan, Luis F. ^[1] ; Freire, Paula P. ^[1] ; Flaws, Jodi A. ^[4] ; Lemos, Bernardo ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Morphol, BR-18618689 Botucatu, SP - Brazil

^[2] Harvard TH Chan Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02115 - USA

^[3] Harvard TH Chan Sch Publ Hlth, Mol & Integrat Physiol Sci Program, Boston, MA 02115 - USA

^[4] Univ Illinois, Dept Comparat Biosci, Champaign, IL 61801 - USA

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGICAL SCIENCES; v. 171, n. 1, p. 84-97, SEP 2019.
• Citações Web of Science: 0

Resumo

Environmental exposure to phthalates during intrauterine development might increase susceptibility to neoplasms in reproductive organs such as the prostate. Although studies have suggested an increase in prostatic lesions in adult animals submitted to perinatal exposure to phthalates, the molecular pathways underlying these alterations remain unclear. Genome-wide levels of mRNAs and miRNAs were monitored with RNA-seq to determine if perinatal exposure to a phthalate mixture in pregnant rats is capable of modifying gene expression during prostate development of the filial generation. The mixture contains diethyl-phthalate, di-(2-ethylhexyl)-phthalate, dibutyl-phthalate, di-isononyl-phthalate, di-isobutyl-phthalate, and benzylbutyl-phthalate. Pregnant females were divided into 4 groups and orally dosed daily from GD10 to PND21 with corn oil (Control: C) or the phthalate mixture at 3 doses (20 mu g/kg/day: T1; 200 mu g/kg/day: T2; 200 mg/kg/day: T3). The phthalate mixture decreased anogenital distance, prostate weight, and decreased testosterone level at the lowest exposure dose at PND22. The mixture also increased inflammatory foci and focal hyperplasia incidence at PND120. miR-184 was upregulated in all treated groups in relation to control and miR-141-3p was only upregulated at the lowest dose. In addition, 120 genes were deregulated at the lowest dose with several of these genes related to developmental, differentiation, and oncogenesis. The data indicate that phthalate exposure at lower doses can cause greater gene expression modulation as well as other downstream phenotypes than exposure at higher doses. A significant fraction of the downregulated genes were predicted to be targets of miR-141-3p and miR-184, both of which were induced at the lower exposure doses. (AU)

• Processo FAPESP: 17/22275-2 - Programação oncotoxicológica pela exposição materna à mistura de diferentes ftalatos: integração do perfil de expressão de miRNAs e mRNAs para reconstrução de redes de regulação gênica associadas à oncogênese
• Beneficiário: Wellerson Rodrigo Scarano
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 17/08306-2 - Programação oncotoxicológica pela exposição materna à mistura de diferentes ftalatos: efeito multigeracional sobre a próstata de ratos e aspectos gênicos e epigenéticos relacionados com o adenocarcinoma prostático
• Beneficiário: Wellerson Rodrigo Scarano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/50002-3 - A collaborative study between Brazilian and US scientists: the effects of environmental chemical exposures on reproduction
• Beneficiário: Wellerson Rodrigo Scarano
• Modalidade de apoio: Auxílio à Pesquisa - Regular