Sulfide-driven denitrification: detecting active microorganisms in fed-batch enrichment cultures by DNA stable isotope probing

Autor(es)

Saia, Flavia Talarico ^[1] ; Octavio de Souza, Theo Syrto ^[2] ; Pozzi, Eloisa ^[3] ; Delgado Duarte, Rubens Tadeu ^[4] ; Foresti, Eugenio ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Sea Sci, Ave Dr Carvalho de Mendonca 144, BR-11070100 Santos, SP - Brazil

^[2] Univ Sao Paulo, Polytech Sch, Dept Hydraul & Environm Engn, Cidade Univ, Ave Prof Almeida Prado 83, Trav 2, BR-05508900 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Engn Sch Sao Carlos, Dept Hydraul & Sanitat, Lab Biol Proc, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[4] Univ Fed Santa Catarina, Dept Microbiol Immunol & Parasitol, Campus Trindade, POB 476, BR-88040900 Florianopolis, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BIOLOGY REPORTS; v. 46, n. 5, p. 5309-5321, OCT 2019.
• Citações Web of Science: 0

Resumo

A microbial community was enriched in the anoxic compartment of a pilot-scale bioreactor that was operated for 180 days, fed with sewage and designed for organic matter, nitrogen and sulfide removal by coupling anaerobic digestion, nitrification and mixotrophic denitrification. Denitrification occurred with endogenous electron donors, mainly sulfide and residual organic matter, coming from the anaerobic compartment. The microorganisms involved in denitrification with sulfide as electron donor were identified by DNA-stable isotope probing with {[}U-C-13]-labelled CO2 and NaHCO3. Complete denitrification occurred every two days, and the applied NO3-/S2- ratio was 1.6. Bacteria belonging to the Sulfurimonas denitrificans was identified as a chemoautotrophic denitrifier, and those related to Georgfuchisa toluolica, Geothrix fermentans and Ferritrophicum radicicola were most probably associated with heterotrophic denitrification using endogenous cells and/or intermediate metabolites. This study showed that DNA-SIP was a suitable technique to identify the active microbiota involved in sulfide-driven denitrification in a complex environment, which may contribute to improve design and operation of bioreactors aiming for carbon-nitrogen-sulfur removal. (AU)

• Processo FAPESP: 10/01735-6 - Utilização da Técnica do DNA-Stable Isotope Probing (DNA-SIP) na identificação de microrganismos desnitrificantes autotróficos em sistema de tratamento de esgoto sanitário utilizando sulfeto como doador de elétrons
• Beneficiário: Flávia Talarico Saia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado