Morphological and molecular characterization of Stigonema jureiensis sp. nov. (Nostocales, Cyanobacteria) from the Atlantic Rainforest, Sao Paulo, Brazil

Autor(es)

Hentschke, Guilherme S. ^[1] ; Rigonato, Janaina ^[2] ; Genuario, Diego B. ^[3] ; Laughinghouse, Haywood D. ^[4] ; Sant'Anna, Celia L. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Porto, Interdisciplinary Ctr Marine & Environm Res CIIMA, Av Gen Norton de Matos S-N, P-4450208 Matosinhos - Portugal

^[2] UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Zool & Bot, R Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Embrapa Environm, Lab Environm Microbiol, BR-13820000 Jaguariuna, SP - Brazil

^[4] Univ Florida IFAS, Agron Dept, Ft Lauderdale Res & Educ Ctr, 3205 Coll Ave, Davie, FL 33314 - USA

^[5] Inst Bot, Lab Phycol, Av Miguel Stefano 3687, BR-04301902 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Fottea; v. 19, n. 2, p. 185-191, 2019.
• Citações Web of Science: 0

Resumo

Tropical regions are considered hotspots of biodiversity, and the genus Stigonema has been underexplored in all world, mainly due to the difficulties of culturing. In this study, we sequenced the 16S rRNA gene of a natural Stigonema population from the tropical Atlantic Rainforest (Sao Paulo, Brazil) and describe S. jureiensis sp. nov. based on phylogenetic and morphological data. For the molecular analysis, the genomic DNA was isolated from aliquots of the population, which were previously manually cleaned under a microscope, using a point-tapered Pasteur pipette to remove other organisms and debris. For morphological analysis, 30 individuals were analyzed and photographed using microscope. Both maximum likelihood and Bayesian phylogenies support S. jureiensis as a new species, and morphological analysis revealed the presence of a special type of mosaic ornate sheaths. (AU)

• Processo FAPESP: 14/26131-7 - Análise do microbioma do Rio Amazonas e seus afluentes: biogeografia e sua contribuição na fixação do N2
• Beneficiário: Diego Bonaldo Genuário
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado