Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse

Autor(es)

de Almeida Scarcella, Ana Silvia ^[1] ; Somera, Alexandre Favarin ^[2] ; Carreira Nunes, Christiane da Costa ^[3] ; Gomes, Eleni ^[3] ; Vici, Ana Claudia ^[2] ; Buckeridge, Marcos Silveira ^[4] ; de Moraes Polizeli, Maria de Lourdes Teixeira ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Bandeirantes Av 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Bandeirantes Av 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Cristovao Colombo St 2265, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[4] Univ Sao Paulo, Inst Biociencias, Dept Bot, Lab Fisiol Ecol LAFIECO, Matao St 277, Cidade Univ, BR-05508090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 24, n. 19 OCT 2019.
• Citações Web of Science: 0

Resumo

Statistical evidence pointing to the very soft change in the ionic composition on the surface of the sugar cane bagasse is crucial to improve yields of sugars by hydrolytic saccharification. Removal of Li+ by pretreatments exposing -OH sites was the most important factor related to the increase of saccharification yields using enzyme cocktails. Steam Explosion and Microwave:H2SO4 pretreatments produced unrelated structural changes, but similar ionic distribution patterns. Both increased the saccharification yield 1.74-fold. NaOH produced structural changes related to Steam Explosion, but released surface-bounded Li+ obtaining 2.04-fold more reducing sugars than the control. In turn, the higher amounts in relative concentration and periodic structures of Li+ on the surface observed in the control or after the pretreatment with Ethanol:DMSO:Ammonium Oxalate, blocked -OH and O- available for ionic sputtering. These changes correlated to 1.90-fold decrease in saccharification yields. Li+ was an activator in solution, but its presence and distribution pattern on the substrate was prejudicial to the saccharification. Apparently, it acts as a phase-dependent modulator of enzyme activity. Therefore, no correlations were found between structural changes and the efficiency of the enzymatic cocktail used. However, there were correlations between the Li+ distribution patterns and the enzymatic activities that should to be shown. (AU)

• Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático