Detection and molecular characterization of Frog virus 3 in bullfrogs from frog farms in Brazil

Autor(es)

Oliveira, Cinthia Rodrigues ^[1] ; Alfaia, Sthefany Rosa ^[1] ; Ikari, Fernanda Lie ^[1] ; Tavares, Loiane Sampaio ^[2] ; Moro de Sousa, Ricardo Luiz ^[2] ; Harakava, Ricardo ^[3] ; Ferreira, Claudia Maris ^[1]

Afiliação do(s) autor(es)

^[1] SAA, APTA, Fisheries Inst, Av Francisco Matarazzo 455, BR-05001900 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, FZEA, BR-13635900 Pirassununga, SP - Brazil

^[3] SAA, Biol Inst, APTA, BR-04014900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Aquaculture; v. 516, FEB 1 2020.
• Citações Web of Science: 0

Resumo

The objective of this study was to perform the molecular diagnosis of ranavirus Frog virus 3 (FV3) in bullfrogs (Lithobates catesbeianus) obtained from frog farms in the central-southern region of Brazil. From each frog farm, we collected adult animals with a mean weight of 120 g +/- 8.0 and tadpoles with a mean weight of 7 g +/- 1.2, totaling 235 animals. For the diagnosis, liver, kidney and spleen samples were collected from the animals and subjected to DNA extraction and conventional PCR. Positive samples were detected on one farm in the state of Sao Paulo. To confirm the results, we performed nucleotide sequencing of the MCP gene and phylogenetic analysis. The results revealed that FV3 strains are present in Brazil with their own phylogenetic profile, and that these strains differ from those affecting amphibians and reptiles described so far in other countries. Further studies are necessary to better understand the circulation of this pathogen in aquatic systems and the dynamics of the dissemination of this disease in Brazil. (AU)

• Processo FAPESP: 15/24590-7 - Detecção e caracterização fenogenotípica de ranavírus (Frog Virus 3) em ranários comerciais paulistas e avaliação da suscetibilidade de rãs-touro (Lithobates catesbeianus) à infecção experimental
• Beneficiário: Cláudia Maris Ferreira Mostério
• Modalidade de apoio: Auxílio à Pesquisa - Regular