Antifungal potential of secondary metabolites involved in the interaction between citrus pathogens

Autor(es)

Costa, Jonas Henrique ^[1] ; Wassano, Cristiane Izumi ^[1] ; Figueiredo Angolini, Celio Fernando ^[2] ; Scherlach, Kirstin ^[3] ; Hertweck, Christian ^{[3, 4]} ; Fill, Taicia Pacheco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Chem, CP 6154, BR-13083970 Campinas, SP - Brazil

^[2] Fed Univ ABC, Ctr Nat & Human Sci, BR-09210580 Santo Andre, SP - Brazil

^[3] Leibniz Inst Nat Prod Res & Infect Biol, Hans Knoll Inst, Dept Biomol Chem, Jena - Germany

^[4] Friedrich Schiller Univ Jena, Chair Nat Prod Chem, D-07743 Jena - Germany

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, DEC 9 2019.
• Citações Web of Science: 0

Resumo

Numerous postharvest diseases have been reported that cause substantial losses of citrus fruits worldwide. Penicillium digitatum is responsible for up to 90% of production losses, and represent a problem for worldwide economy. In order to control phytopathogens, chemical fungicides have been extensively used.Yet, the use of some artificial fungicides cause concerns about environmental risks and fungal resistance. Therefore, studies focusing on new approaches, such as the use of natural products, are getting attention. Co-culture strategy can be applied to discover new bioactive compounds and to understand microbial ecology. Mass Spectrometry Imaging (MSI) was used to screen for potential antifungal metabolites involved in the interaction between Penicillium digitatum and Penicillium citrinum. MSI revealed a chemical warfare between the fungi: two tetrapeptides, deoxycitrinadin A, citrinadin A, chrysogenamide A and tryptoquialanines are produced in the fungi confrontation zone. Antimicrobial assays confirmed the antifungal activity of the investigated metabolites. Also, tryptoquialanines inhibited sporulation of P. citrinum. The fungal metabolites reported here were never described as antimicrobials until this date, demonstrating that co-cultures involving phytopathogens that compete for the same host is a positive strategy to discover new antifungal agents. However, the use of these natural products on the environment, as a safer strategy, needs further investigation. This paper aimed to contribute to the protection of agriculture, considering health and ecological risks. (AU)

• Processo FAPESP: 19/06359-7 - Compreensão dos fatores químicos envolvidos no endofitismo x patogenicidade de diferentes isolados de Penicillium brasilianum
• Beneficiário: Taicia Pacheco Fill
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/24462-4 - Entendendo as estratégias de virulência do fungo Penicillium digitam contra seu hospedeiro citros
• Beneficiário: Taicia Pacheco Fill
• Modalidade de apoio: Auxílio à Pesquisa - Regular