Cooling of in vitro-produced bovine embryos: Effects of medium and time on gene expression, DNA fragmentation and embryonic survival

Autor(es)

Marqui, Fernanda N. ^[1] ; Martins, Jr., Alicio ^[2] ; Cardoso, Tereza C. ^[3] ; Cruz, Tairini E. ^[2] ; Dell'Aqua, Jr., Jose Antonio ^[1] ; Oba, Eunice ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Rubiao Jr S-N, BR-18618970 Botucatu, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Sch Vet Med, Dept Clin Surg & Anim Reprod, BR-16050680 Aracatuba, SP - Brazil

^[3] UNESP Sao Paulo State Univ, Sch Vet Med, Dept Support Prod & Anim Hlth, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIVESTOCK SCIENCE; v. 230, DEC 2019.
• Citações Web of Science: 0

Resumo

The aim of this study was to investigate the effects of Medium 199 (M199) and BotuEmbryo (R) (BE) as well as the time (24 an 48 h) of cooling at 5 degrees C on the transcript level of genes related to bovine embryonic metabolism (GLUM and cellular stress (HSPA1A, PRDX1 and SOD1), on the rate of blastocyst re-expansion and hatch, embryo survival, total number of cells and DNA fragmentation in IVP bovine embryos. There was an overall reduction in embryo survival and development to hatching/hatched stage with the increase in cooling time, especially in those cooled in M199. Although TUNEL-positive cells were identified in both cooled and NC embryos, a higher number of apoptotic cells was observed in BE-48 than in NC and BE-24. However, no effect of either medium or cooling time was found among all groups for the set of gene transcripts. In conclusion, no detrimental effect on the expression of genes as well as embryonic cell proliferation was remarkable in cooling of IVP bovine embryos. However, according to the in vitro predictive assessment of embryo viability, the cooling of IVP bovine embryos in the evaluated media appears to be feasible for 24 h but not for 48 h. (AU)

• Processo FAPESP: 13/15905-9 - Efeitos de diferentes meios de cultivo, temperaturas e tempos de refrigeração sobre a viabilidade e expressão gênica de embriões bovinos produzidos in vitro
• Beneficiário: Fernanda Nunes Marqui
• Modalidade de apoio: Bolsas no Brasil - Mestrado