Detection of the Prostate Cancer Biomarker PCA3 with Electrochemical and Impedance-Based Biosensors

Autor(es)

Soares, Juliana Coatrini ^{[1, 2]} ; Soares, Andrey Coatrini ^{[1, 2]} ; Rodrigues, Valquiria Cruz ^[1] ; Melendez, Matias Eliseo ^[3] ; Santos, Alexandre Cesar ^[3] ; Faria, Eliney Ferreira ^[3] ; Reis, Rui M. ^{[4, 3, 5]} ; Carvalho, Andre Lopes ^[3] ; Oliveira, Jr., Osvaldo N. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Carlos, SP - Brazil

^[2] Embrapa Instrumentat, Natl Lab Nanotechnol Agribusiness LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos - Brazil

^[4] ICVS 3Bs PT Govt Associate Lab, Braga - Portugal

^[5] Univ Minho, Sch Med, Life & Hlth Sci Res Inst ICVS, Braga - Portugal

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 11, n. 50, p. 46645-46650, DEC 18 2019.
• Citações Web of Science: 0

Resumo

Diagnosis of prostate cancer via PCA3 biomarker detection is promising to be much more efficient than with the prostatic specific antigens currently used. In this study, we present the first electrochemical and impedance-based biosensors that are capable of detecting PCA3 down to 0.128 nmol/L. The biosensors were made with a layer of PCA3-complementary single-stranded DNA (ssDNA) probe, immobilized on a layer-by-layer (LbL) film of chitosan (CHT) and carbon nanotubes (MVVCNT). They are highly selective to PCA3, which was confirmed in impedance measurements and with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). Using information visualization methods, we could also distinguish between cell lines expressing the endogenous PCA3 long noncoding RNA (lncRNA) from cells that did not contain detectable levels of this biomarker. Since the methods involved in fabrication the biosensors are potentially low cost, one may hope to deploy PCA3 tests in any laboratory of clinical analyses and even for point-of-care diagnostics. (AU)

• Processo FAPESP: 18/18953-8 - Filmes nanoestruturados aplicados em biossensores microfluídicos para detecção de mastite bacteriana
• Beneficiário: Andrey Coatrini Soares
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático