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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Designing biocompatible and multicolor fluorescent hydroxyapatite nanoparticles for cell-imaging applications

Texto completo
Machado, T. R. [1] ; Leite, I. S. [2] ; Inada, N. M. [2] ; Li, M. S. [2] ; da Silva, J. S. [1] ; Andres, J. [3] ; Beltran-Mir, H. [4] ; Cordoncillo, E. [4] ; Longo, E. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos UFSCar, Dept Quim, CDMF, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[3] Univ Jaume 1, Dept Quim Fis & Analit 1, Castellon de La Plana 12071 - Spain
[4] Univ Jaume 1, Dept Quim Inorgan & Organ, Castellon de La Plana 12071 - Spain
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Citações Web of Science: 1

In recent years, there has been a growing effort toward the synthesis, engineering and property tuning of biocompatible nanoparticles (NPs) that can be detected by confocal microscopy and then used as fluorescent probes. Defect-related fluorescent hydroxyapatite (HA) is attracting considerable attention as a suitable material for cell-imaging owing to its excellent biocompatibility, biodegradability, easy cell internalization capability, and its stable and intense blue fluorescence. Although the self-activated fluorescence of HA is advantageous, as it avoids the use of lanthanide dopants, organic dyes, or the need to be combined with other fluorescent inorganic nanocrystals, its preparation by simple procedures with fine control of the defects which govern this property remains challenging. In this study, we propose a new, simple, and cost-effective strategy of fluorescence imaging using HA nanorods (HAnrs) obtained by chemical precipitation followed by heat treatment at relative low temperature (350 degrees C) without using any sophisticated equipment or inorganic/organic additives. Structural, compositional, and morphological analysis, as well as a cytotoxicity assay, are described in detail. The fluorescence characterization of HAnrs shows an intense bluish-white broad-band emission (lambda(max) = 535 nm), and the defects which cause this behavior are studied by temperature-dependent photoluminescence measurements (38-300 K). Moreover, the high density of defects in heat-treated HA leads to tunable fluorescent property (lambda(max) = 399-650 nm) across the entire visible spectrum as a function of the excitation wavelength (lambda(exc) = 330-630 nm) with the potential for further multicolor imaging applications. Labeling results by confocal microscopy show that HAnr co-cultured with human dermal fibroblast cell line exhibit fluorescence signals in the cells even after 48 h of incubation with no evident cytotoxic effects. Therefore, heat-treated fluorescent HAnr can be utilized for tracking and monitoring cells and is a safe alternative for the traditional probes used in bioimaging procedures. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
Beneficiário:Vanderlei Salvador Bagnato
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA
Beneficiário:Igor Polikarpov
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 13/11144-3 - Estudo, Caracterização e Propriedades de Ortofosfatos Multifuncionais
Beneficiário:Thales Rafael Machado
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
Beneficiário:Elson Longo da Silva
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs