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Sublethal effects of waterborne copper and copper nanoparticles on the freshwater Neotropical teleost Prochilodus lineatus: A comparative approach

Texto completo
Autor(es):
Tesser, Maria Eduarda [1] ; de Paula, Angelica Alves [1] ; Risso, Wagner Ezequiel [1] ; Monteiro, Renata Aparecida [2] ; Santo Pereira, Anderson do Espirito [2] ; Fraceto, Leonardo Fernandes [2] ; dos Reis Martinez, Claudia Bueno [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] State Univ Londrina UEL, Dept Physiol Sci, Londrina, Parana - Brazil
[2] Sao Paulo State Univ Unesp, Inst Sci & Technol, Sorocaba - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Science of The Total Environment; v. 704, FEB 20 2020.
Citações Web of Science: 0
Resumo

Copper nanoparticles can contaminate the aquatic environment, but their effects on fish and how they may differ from copper salts is not understood. Thus, in this work we compare the sublethal effects of copper nanoparticles (nCu) and copper chloride (Cu) on the freshwater teleost Prochilodus lineatus, known for its sensitivity to copper. Juveniles (n = 8/group) were exposed to 20 mu g L-1 of copper as CuCl2 (Cu), 40 mu g L-1 of copper nanoparticles (nCu), or only water (control), for 96 h. These concentrations were chosen to achieve similar dissolved copper concentration in both treatments (Cu: 10.29 +/- 0.94 mu g L-1; nCu: 12.16 +/- 1.77 mu g L-1). After the exposure, the following biological parameters were evaluated: copper accumulation in the gills, liver, gastrointestinal tract, kidney, and muscle; hematocrit (Ht) and hemoglobin content (Hb); branchial activity of Na+-K+-ATPase (NaKATP), H+-ATPase (HATP), Ca-2(+)-ATPase (CaATP), and carbonic anhydrase (CA); glutathione content (GSH) and lipid peroxidation (LPO) in the liver; acetylcholinesterase activity (AChE) in the brain and muscle; and histopathology of the gills and liver. The gills of Cu-exposed fish were adversely affected, with increased copper content, inhibition of H+-ATPase and Ca2+-ATPase, and histological damage, including proliferation of mitochondria rich cells and/or mucous cells. In addition, LPO levels increased in the liver of Cu-exposed fish, indicating the occurrence of oxidative stress. Exposure to nCu promoted a decrease in Ht and Hb, indicating anemia, and an increase in branchial Na+-K+-ATPase and H+-ATPase activities, which can be an adaptive response to metabolic acidosis. Within the chosen biomarkers and the conditions tested, copper nanoparticles were less toxic than copper. However, the effects promoted by the nanoparticles were different from those promoted by copper. These results emphasize the need for a better understanding of copper nanoparticles toxicity in order to establish safe concentrations and avoid environment impacts. (C) 2019 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 17/21004-5 - Agricultura, micro/nanotecnologia e ambiente: da avaliação dos mecanismos de ação a estudos de transporte e toxicidade
Beneficiário:Leonardo Fernandes Fraceto
Linha de fomento: Auxílio à Pesquisa - Temático