Damage and drying modify the composition of Mikania glomerata and Mikania laevigata leaves

Autor(es)

Borghi, Alexandre A. ^[1] ; Almeida, Claudia de L. ^[1] ; Sawaya, Alexandra C. H. F. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Ciencias Farmaceut, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 29, n. 6, p. 793-797, NOV-DEC 2019.
• Citações Web of Science: 0

Resumo

This study compared the influence of mechanical damage and drying on the chemical composition of Mikania glomerata Spreng. and Mikania laevigata Sch.Bip. ex Baker, Asteraceae, leaves. Leaves were collected 1-24 h after damage. Oven-drying at 40 degrees C and shade-drying at ambient temperature were compared to lyophilization. Samples were extracted in 70% ethanol and analyzed by ultra-high-performance liquid chromatography with mass spectrometry. Significant (p< 0.05) increases of caffeoylquinic acids were observed in damaged leaves of both species and coumarin decreased in M. laevigata, indicating stress. Although the final water content was similar, the drying method affected the leaf composition. In shade-dried leaves of M. laevigata coumarin decreased and the presence of umbelliferone was observed; caffeoylquinic acid contents increased for 288 h in in both species. Apparently, enzymes were inactivated after 6 h in oven drying, stabilizing their chemical composition, while shade drying allowed enzymatic and microbial activity to continue; illustrating the importance of post harvesting procedures on the quality of medicinal plants. (C) 2019 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda. (AU)

• Processo FAPESP: 13/15962-2 - Metabólitos secundários de duas espécies de guaco (Mikania glomerata Sprenguel e Mikania laevigata Schultz) cultivadas sob condições variadas.
• Beneficiário: Claudia de Lazzari Almeida
• Linha de fomento: Bolsas no Brasil - Mestrado