Transcriptional patterns of Coffea arabica L. nitrate reductase, glutamine and asparagine synthetase genes are modulated under nitrogen suppression and coffee leaf rust

Autor(es):Mostrar menos -

Baba, Viviane Yumi ^{[1, 2]} ; Braghini, Masako Toma ^[3] ; dos Santos, Tiago Benedito ^{[1, 4]} ; de Carvalho, Kenia ^{[1, 5]} ; Moura Soares, Joao Danillo ^[1] ; Ivamoto-Suzuki, Suzana Tiemi ^{[1, 6]} ; Maluf, Mirian P. ^{[7, 3]} ; Padilha, Lilian ^{[7, 3]} ; Paccola-Meirelles, Luzia D. ^{[2, 8]} ; Pereira, Luiz Filipe ^{[1, 7]} ; Domingues, Douglas S. ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Inst Agron Parana, Plant Biotechnol Lab, Londrina, Parana - Brazil

^[2] Univ Estadual Londrina, Dept Agron, Londrina, Parana - Brazil

^[3] Inst Agron Campinas, Ctr Anal & Pesquisa Tecnol Agronegocio Cafe Alcid, Campinas, SP - Brazil

^[4] Univ Oeste Paulista, Programa Posgrad Agron, Presidente Prudente, SP - Brazil

^[5] Embrapa Soja, Plant Biotechnol Lab, Londrina, Parana - Brazil

^[6] Sao Paulo State Univ, UNESP, Inst Biociencias, Dept Bot, Rio Claro, SP - Brazil

^[7] Embrapa Cafe, Plant Breeding, Brasilia, DF - Brazil

^[8] Univ Paranaense, Dept Agron, Umuarama, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PeerJ; v. 8, JAN 3 2020.
• Citações Web of Science: 3

Resumo

This study evaluated the transcriptional profile of genes related to nitrogen (N) assimilation in coffee plants susceptible and resistant to rust fungi under N sufficiency and N suppression. For this purpose, we inoculated young coffee leaves with Hemileia vastatrix uredospores and collected them at 0, 12, 24 and 48 hours post-inoculation (HPI) to evaluate the relative expressions of genes encoding cytosolic glutamine synthetase (CaGS(1 )), plastid glutamine synthetase (CaGS(2) ), nitrate reductase (CaNR), and asparagine synthetase (CaAS). The genes exhibited distinct patterns of transcriptional modulation for the different genotypes and N nutritional regimes. The resistant genotype (I59) presented high levels of transcription in response to pathogen inoculation for CaNR and CaGS(1), genes, evaluated under N sufficiency in the initial moments of infection (12 HPI). The gene CaGS(1) also showed a peak at 48 HPI. The susceptible genotype (CV99) showed increased transcript rates of CaNR at 12 and 24 HPI in response to rust inoculation. The transcriptional patterns observed for CV99, under N suppression, were high levels for CaAS and CaGS(2) at all post-inoculation times in response to coffee leaf rust disease. In addition, CaGS(1) was up-regulated at 48 HPI for CV99. Cultivar I59 showed high transcript levels at 12 HPI for CaAS and peaks at 24 and 48 HPI for CaGS(2) in inoculated samples. Consequently, total chlorophyl concentration was influenced by N suppression and by rust infection. Regarding enzyme activities in vitro for glutamine synthetase and CaNR, there was an increase in infected coffee leaves (159) and under N sufficiency. Moreover, CV99 was modulated in both N nutritional regimes for GS activity in response to rust. Our results indicate that N transport genes trigger a differential modulation between genotypes through the action of rust disease. (AU)

• Processo FAPESP: 17/01455-2 - Bases bioquímicas e moleculares da rede metabólica de diterpenos, compostos bioativos e naturais de cafeeiros
• Beneficiário: Suzana Tiemi Ivamoto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/10896-0 - A sinalização do ácido hexanóico pode modular o transcriptoma, metaboloma e o desenvolvimento de patógenos no cafeeiro?
• Beneficiário: Douglas Silva Domingues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores