Graphene oxide-based nanomaterial interaction with human breast cancer cells

Autor(es)

Ribeiro, Beatriz F. M. ^[1] ; Souza, Marcelo M. ^[1] ; Fernandes, Daniela S. ^{[2, 3]} ; do Carmo, Devaney R. ^[2] ; Machado-Santelli, Glaucia M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo, SP - Brazil

^[2] Univ Estadual Paulista, Dept Phys & Chem, Sao Paulo, SP - Brazil

^[3] Fac Philosophy Sci & Arts Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biomedical Materials Research Part A; v. 108, n. 4 DEC 2019.
• Citações Web of Science: 0

Resumo

Graphene and graphene-based nanomaterials have great potential for various biomedical applications due to their unique physicochemical properties. However, how graphene-based nanomaterials interact with biological systems has not been thoroughly studied. This study shows that 24, 48, and 72 hr exposure of 2.4 mu g/cm(2) of graphene oxide (GOX) and GOX modified with DAB-AM-16 and PAMAM dendrimers (GOXD and GOXP, respectively) did not exhibit toxicity to MCF-7 cells. However, higher graphene concentrations, such as 24 and 48 mu g/cm(2), induced low cytotoxic effects. The GOX, GOXD, and GOXP particles have a strong affinity with the cellular membrane. Cells that internalized the nanomaterials presented morphological alterations and modifications in the organization of microfilaments and microtubules compared with control cells. Then, cells were treated with 24 mu g/cm(2) of GOX, GOXD or GOXP for 24 hr and recovered for an additional period of 24 hr in normal medium. Nanoparticles remained in the cytoplasm of some cells, apparently with no effect on cellular morphology, being consistent with the data found in the cell proliferation experiment, which showed that the cells remained alive up to 72 hr. (AU)

• Processo FAPESP: 18/22882-9 - Interiorização de óxido de grafeno por células tumorais em cultura: avaliação dos mecanismos e efeitos
• Beneficiário: Beatriz Fumelli Monti Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/16893-5 - Estudo dos efeitos do acúmulo de óxido de grafeno sobre a rede de microfilamentos e microtúbulos na linhagem celular MCF7
• Beneficiário: Beatriz Fumelli Monti Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/20397-8 - Modificação da superfície do grafeno com um dendrímero: Um estudo de materiais híbridos nanoestruturados para aplicações químicas e biológicas
• Beneficiário: Devaney Ribeiro Do Carmo
• Modalidade de apoio: Auxílio à Pesquisa - Regular