Bacterial diversity in deep-sea sediments under influence of asphalt seep at the Sao Paulo Plateau

Autor(es):Mostrar menos -

Queiroz, Luciano Lopes ^{[1, 2]} ; Bendia, Amanda Goncalves ^[1] ; Delgado Duarte, Rubens Tadeu ^[3] ; das Gracas, Diego Assis ^[4] ; da Costa da Silva, Artur Luiz ^[4] ; Nakayama, Cristina Rossi ^[5] ; Sumida, Paulo Yukio ^[1] ; Lima, Andre O. S. ^[6] ; Nagano, Yuriko ^[7] ; Fujikura, Katsunori ^[7] ; Kitazato, Hiroshi ^[7] ; Pellizari, Vivian Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Oceanog, Praca Oceanog 191, BR-05508120 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Microbiol Grad Program, Sao Paulo - Brazil

^[3] Univ Fed Santa Catarina, Microbiol Immunol & Parasitol Dept, CCB MIP, Campus Trindade, POB 476, BR-88040900 Florianopolis, SC - Brazil

^[4] Fed Univ Para, Inst Biol Sci, Rua Augusto Correa 01, BR-66075110 Belem, Para - Brazil

^[5] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Rua Sao Nicolau 210, BR-09913030 Diadema - Brazil

^[6] Univ Vale Itajai, Ctr Ciencias Tecnol Terra & Mar CTTMAR, Rua Uruguai 458, BR-88302202 Itajai, SC - Brazil

^[7] Japan Agcy Marine Earth Sci & Technol, 2-15 Natsushima, Yokosuka, Kanagawa 2370061 - Japan

• Tipo de documento: Artigo Científico
• Fonte: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 113, n. 5 JAN 2020.
• Citações Web of Science: 0

Resumo

Here we investigated the diversity of bacterial communities from deep-sea surface sediments under influence of asphalt seeps at the Sao Paulo Plateau using next-generation sequencing method. Sampling was performed at North Sao Paulo Plateau using the human occupied vehicle Shinkai 6500 and her support vessel Yokosuka. The microbial diversity was studied at two surficial sediment layers (0-1 and 1-4 cm) of five samples collected in cores in water depths ranging from 2456 to 2728 m. Bacterial communities were studied through sequencing of 16S rRNA gene on the Ion Torrent platform and clustered in operational taxonomic units. We observed high diversity of bacterial sediment communities as previously described by other studies. When we considered community composition, the most abundant classes were Alphaproteobacteria (27.7%), Acidimicrobiia (20%), Gammaproteobacteria (11.3%) and Deltaproteobacteria (6.6%). Most abundant OTUs at family level were from two uncultured bacteria from Actinomarinales (5.95%) and Kiloniellaceae (3.17%). The unexpected high abundance of Alphaproteobacteria and Acidimicrobiia in our deep-sea microbial communities may be related to the presence of asphalt seep at North Sao Paulo Plateau, since these bacterial classes contain bacteria that possess the capability of metabolizing hydrocarbon compounds. (AU)

• Processo FAPESP: 13/09159-2 - Comunidades de micro-organismos extremófilos em ambientes de mar profundo do Atlântico Sul
• Beneficiário: Luciano Lopes Queiroz
• Modalidade de apoio: Bolsas no Brasil - Mestrado