Is the profile of transcripts altered in the eutopic endometrium of infertile women with endometriosis during the implantation window?

Autor(es)

Da Broi, M. G. ^[1] ; Meola, J. ^[1] ; Placa, J. R. ^[2] ; Peronni, K. C. ^[3] ; Rocha Jr, V, C. ; Silva Jr, W. A. ; Ferriani, R. A. ^{[4, 5]} ; Navarro, P. A. ^{[4, 5]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Div Human Reprod, Dept Gynecol & Obstet, Ribeirao Preto Med Sch, Ave Bandeirantes 900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] NAP USP, Ctr Integrat Syst Biol CISBi, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ctr Med Genom, HCFMRP, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Rocha Jr, Jr., C., Univ Sao Paulo, Div Human Reprod, Dept Gynecol & Obstet, Ribeirao Preto Med Sch, Ave Bandeirantes 900, BR-14049900 Ribeirao Preto, SP - Brazil

^[5] Natl Council Sci & Technol Dev CNPq, Natl Inst Hormones & Womans Hlth, Porto Alegre, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Human Reproduction; v. 34, n. 12, p. 2381-2390, DEC 2019.
• Citações Web of Science: 0

Resumo

STUDY QUESTION: Compared to healthy women, is the profile of transcripts altered in the eutopic endometrium of infertile women with endometriosis during the implantation window (IW)? SUMMARY ANSWER: The eutopic endometrium of infertile women with endometriosis seems to be transcriptionally similar to the endometrium of infertile and fertile controls (FC) during the IW. WHAT IS KNOWN ALREADY: Endometriosis is a disease related to infertility; nevertheless, little is known regarding the ethiopathogenic mechanisms underlying this association. Some studies evaluating the eutopic endometrium of endometriosis patients suggest there is an endometrial factor involved in the disease-related infertility. However, no study to date has evaluated the endometrial transcriptome (mRNA and miRNA) by next generation sequencing (NGS), comparing patients with endometriosis as the exclusive infertility factor (END) to infertile controls (IC; male and/or tubal factor) and FC. STUDY DESIGN, SIZE, DURATION: From November 2011 to November 2015 we performed a case-control study, where 17 endometrial samples (six END, six IC, five FC) were collected during the IW. PARTICIPANTS/MATERIALS, SETTING, METHODS: All endometrial samples had the RNA extracted. Two libraries were prepared for each one (mRNA and miRNA), which were sequenced, respectively, at HISEQ 2500 (RNA-Seq) and MiSeq System (miRNA-Seq), Illumina. The normalization and differential expression were conducted in statistical R environment using DESeq2 package. qPCR was used for data validation, which were analyzed by Kruskal-Wallis test and Dunn posttest (P <0.05). MAIN RESULTS AND THE ROLE OF CHANCE: RNA-Seq revealed no differentially expressed genes (DEG) among END, IC and FC groups. miRNA-Seq revealed three differentially expressed miRNAs (has-27a-5p, has-miR-150-5p, has-miR-504-5p) in END group compared to FC group. However, none of the miRNAs identified in the sequencing was validated by qPCR. LIMITATIONS, REASONS FOR CAUTION: The main limitation of this study was the small sample size evaluated as a result of the restrictive eligibility criteria adopted, limiting the generalization of the results obtained here. On the other hand, strict eligibility criteria, which eliminated factors potentially related to impaired endometrial receptivity, were required to increase the study's internal validity. WIDER IMPLICATIONS OF THE FINDINGS: This study brings new perspectives on the mechanisms involved in endometriosis-related infertility. The present findings suggest the eutopic endometrium of infertile women with endometriosis, without considering the disease's stage, is transcriptionally similar to controls during the IW, possibly not affecting receptivity. Further studies are needed to evaluate endometrial alterations related to endometriosis' stages. (AU)

• Processo FAPESP: 11/17614-6 - Avaliação do perfil de expressão dos genes PGR, HBEGF, ITGAV, ITGB3 e SPP1 em endométrio eutópico de pacientes inférteis com endometriose e controles
• Beneficiário: Michele Gomes Da Broi
• Modalidade de apoio: Bolsas no Brasil - Doutorado