Genome-wide characterization of the laccase gene family in Setaria viridis reveals members potentially involved in lignification

Autor(es)

Simoes, Marcella Siqueira ^[1] ; Carvalho, Gabriel Garon ^[1] ; Ferreira, Savio Siqueira ^[1] ; Hernandes-Lopes, Jose ^[1] ; de Setta, Nathalia ^[2] ; Cesarino, Igor ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Rua Matao 277, BR-05508090 Sao Paulo - Brazil

^[2] Univ Fed ABC, Ctr Ciencias Nat Humanas E, BR-09606070 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLANTA; v. 251, n. 2 JAN 9 2020.
• Citações Web of Science: 0

Resumo

Plant laccases are copper-containing glycoproteins involved in monolignol oxidation and, therefore, their activity is essential for lignin polymerization. Although these enzymes belong to large multigene families with highly redundant members, not all of them are thought to be involved in lignin metabolism. Here, we report on the genome-wide characterization of the laccase gene family in the model C4 grass Setaria viridis and further identification of the members potentially involved in monolignol oxidation. A total of 52 genes encoding laccases (SvLAC1 to SvLAC52) were found in the genome of S. viridis, and phylogenetic analyses showed that these genes were heterogeneously distributed among the characteristic six subclades of the family and are under relaxed selective constraints. The observed expansion in the total number of genes in this species was mainly caused by tandem duplications within subclade V, which accounts for 68% of the whole family. Comparative phylogenetic analyses showed that the expansion of subclade V is specifically observed for the Paniceae tribe within the Panicoideae subfamily in grasses. Five SvLAC genes (SvLAC9, SvLAC13, SvLAC15, SvLAC50, and SvLAC52) fulfilled the criteria established to identify lignin-related candidates: (1) phylogenetic proximity to previously characterized lignin-related laccases from other species, (2) similar expression pattern to that observed for lignin biosynthetic genes in the S. viridis elongating internode, and (3) high expression in S. viridis tissues undergoing active lignification. In addition, in situ hybridization experiments not only confirmed that these selected SvLAC genes were expressed in lignifying cells, but also that their expression showed different tissue specificities, suggesting subfunctionalization events within the family. These five laccase genes are strong candidates to be involved in lignin polymerization in S. viridis and might be good targets for lignin bioengineering strategies. (AU)

• Processo FAPESP: 15/02527-1 - Desenvolvimento de sistemas-modelo em sorgo e uso da biologia de sistemas na elucidação dos mecanismos moleculares do metabolismo fenólico e de lignina em gramíneas
• Beneficiário: Igor Cesarino
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores
• Processo FAPESP: 16/06917-1 - Análise do transcriptoma e construção de redes de co-expressão em sistemas modelo para identificação de genes envolvidos na deposição de parede celular secundária e no metabolismo de lignina em gramíneas
• Beneficiário: Sávio de Siqueira Ferreira
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/50189-0 - Can we control how plants produce their biomass?
• Beneficiário: Igor Cesarino
• Linha de fomento: Auxílio à Pesquisa - Regular