Hyperbaric oxygenation improves redox control and reduces mortality in the acute phase of myocardial infarction in a rat model

Autor(es)

Oliveira, Mario S. ^[1] ; Tanaka, Leonardo Y. ^[2] ; Antonio, Ednei L. ^[1] ; Brandizzi, I, Laura ; Serra, Andrey J. ^[1] ; Dos Santos, Leonardo ^[3] ; Krieger, Jose E. ^[4] ; Laurindo, Francisco R. M. ^[5] ; Tucci, Paulo J. F. ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Paulo UNIFESP, Div Cardiol, Rua Pedro Toledo 181, BR-04039032 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Heart Inst, Vasc Biol Lab, BR-05403900 Sao Paulo - Brazil

^[3] Fed Univ Espirito Santo UFES, Dept Physiol Sci, BR-29043215 Vitoria, ES - Brazil

^[4] Univ Sao Paulo, Heart Inst, Lab Genet & Mol Cardiol, BR-05403900 Sao Paulo - Brazil

^[5] Brandizzi, Laura, I, Univ Sao Paulo, Heart Inst, Vasc Biol Lab, BR-05403900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR MEDICINE REPORTS; v. 21, n. 3, p. 1431-1438, MAR 2020.
• Citações Web of Science: 0

Resumo

Among the mechanisms of action of hyperbaric oxygenation (HBO), the chance of reducing injury by interfering with the mechanisms of redox homeostasis in the heart leads to the possibility of extending the period of viability of the myocardium at risk. This would benefit late interventions for reperfusion to the ischemic area. The objective of the present study was to investigate the changes in the redox system associated with HBO therapy maintained during the first hour after coronary occlusion in an acute myocardial infarction (MI) rat model. Surviving male rats (n=105) were randomly assigned to one of three groups: Sham (SH=26), myocardial infarction (MI=45) and infarction+hyperbaric therapy (HBO=34, 1 h at 2.5 atm). After 90 min of coronary occlusion, a sample of the heart was collected for western blot analysis of total protein levels of superoxide dismutase, catalase, peroxiredoxin and 3-nitrotyrosine. Glutathione was measured by enzyme-linked immunosorbent assay (ELISA). The detection of the superoxide radical anion was carried out by oxidation of dihydroethidium analyzed with confocal microscopy. The mortality rate of the MI group was significantly higher than that of the HBO group. No difference was noted in the myocardial infarction size. The oxidized/reduced glutathione ratio and peroxiredoxin were significantly higher in the SH and MI when compared to the HBO group. Superoxide dismutase enzymes and catalase were significantly higher in the HBO group compared to the MI and SH groups. 3-Nitrotyrosine and the superoxide radical were significantly lower in the HBO group compared to these in the MI and SH groups. These data demonstrated that hyperbaric oxygenation therapy decreased mortality by improving redox control in the hearts of rats in the acute phase of myocardial infarction. (AU)

• Processo FAPESP: 09/54225-8 - Fisiopatologia da insuficiência cardíaca congestiva
• Beneficiário: Paulo Jose Ferreira Tucci
• Modalidade de apoio: Auxílio à Pesquisa - Temático