Pair Distribution Function from Electron Diffraction in Cryogenic Electron Microscopy: Revealing Glassy Water Structure

Autor(es)

Souza Junior, Joao Batista ^[1] ; Schleder, Gabriel Ravanhani ^{[2, 1]} ; Colombari, Felippe Mariano ^[1] ; de Farias, Marcelo Alexandre ^[1] ; Bettini, Jefferson ^[1] ; van Heel, Marin ^{[1, 3, 4]} ; Portugal, Rodrigo Villares ^[1] ; Fazzio, Adalberto ^{[2, 1]} ; Leite, Edson Roberto ^{[5, 1]}

Afiliação do(s) autor(es)

^[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas - Brazil

^[2] Fed Univ ABC UFABC, BR-09210580 Santo Andre, SP - Brazil

^[3] Ctr Electron Nanoscopy NeCEN, NL-2333 CC Leiden - Netherlands

^[4] Imperial Coll London, Fac Nat Sci, London SW7 2AZ - England

^[5] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Physical Chemistry Letters; v. 11, n. 4, p. 1564-1569, FEB 20 2020.
• Citações Web of Science: 0

Resumo

In recent years, cryogenic electron microscopy (Cryo-EM) has revolutionized the structure determination of wet samples and especially that of biological macromolecules. The glassy-water medium in which the molecules are embedded is considered an almost in vivo environment for biological samples. The local structure of amorphous ice is known from neutron- and X-ray-diffraction studies, techniques appropriate for much larger volumes than those used in cryo-EM. We here present a first study of the pair-distribution function g(r) of glassy water under cryo-EM conditions using electron diffraction data. We found g(r) to be between that of low-density amorphous ice and that of supercooled water. Under electron exposure, cubic-ice regions were found to nucleate in thicker glassy-water samples. Our work enables to obtain quantitative structural information using g(r) from cryo-EM. (AU)

• Processo FAPESP: 17/02317-2 - Interfaces em materiais: propriedades eletrônicas, magnéticas, estruturais e de transporte
• Beneficiário: Adalberto Fazzio
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/18139-6 - Machine learning e Ciência de Materiais: descoberta e design de materiais 2D
• Beneficiário: Gabriel Ravanhani Schleder
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/05159-1 - Auto-organização de nanopartículas inorgânicas utilizando processos estáticos e dinâmicos
• Beneficiário: João Batista Souza Junior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado