Antimicrobial, anti-adherence and antibiofilm activity against Staphylococcus aureus of a 4-phenyl coumarin derivative isolated from Brazilian geopropolis

Autor(es)

da Cunha, Marcos Guilherme ^{[1, 2]} ; Orlandi Sardi, Janaina de Cassia ^[2] ; Freires, Irlan Almeida ^[3] ; Franchin, Marcelo ^[2] ; Rosalen, Pedro Luiz ^[2]

Afiliação do(s) autor(es)

^[1] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, 901 Limeira Ave, BR-13414903 Piracicaba, SP - Brazil

^[3] Univ Florida, Dept Oral Biol, Coll Dent, 1395 Ctr Dr, Gainesville, FL 32610 - USA

• Tipo de documento: Artigo Científico
• Fonte: Microbial Pathogenesis; v. 139, FEB 2020.
• Citações Web of Science: 0

Resumo

The discovery of new drug candidates, especially from natural products, remains a promising approach to overcome the alarmingly high microbial resistance rates. A major 4-phenyl coumarin named cinnamoyloxy-mammeisin (CNM) isolated from stingless bee geopropolis showed interesting biological properties; however, its antimicrobial activity against Staphylococcus aureus has never been investigated. In order to clarify these properties, CNM isolated from geopropolis was initially tested against methicillin-susceptible and -resistant S. aureus strains. Further, the effects of CNM were assessed on the microbial adherence to human cells, biofilm formation and mature biofilm. Then, the acute toxicity of the compound was determined in Galleria mellonella. CNM showed bacteriostatic activity against methicillin-susceptible and -resistant S. aureus strains, with MIC of 11.3 mu M. In addition, CNM at 5.7 mu M reduced bacterial adherence to human keratinocytes from 1 to 3 h and disrupted biofilm formation by reducing cell viability and architecture, as evidenced by scanning electron microscopy. The acute toxicity assay indicated no significant harmful effects. Based on these findings, CNM can be considered a promising compound with anti-S. aureus properties and predicted low toxicity. Thus, it may be used as a drug candidate or lead compound for structure/activity optimization. (AU)

• Processo FAPESP: 11/23635-6 - Avaliação farmacológica e isolamento de moléculas bioativas da fração hexânica da geoprópolis de melipona scutellaris
• Beneficiário: Marcos Guilherme da Cunha
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/22002-2 - Identificação química de biocompostos e alvos moleculares da geoprópolis sobre células cancerosas
• Beneficiário: Marcos Guilherme da Cunha
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado