Protective effect of N-acetylcysteine on the toxicity of silver nanoparticles: Bioavailability and toxicokinetics in Enchytraeus crypticus

Autor(es)

Padilha Mendonca, Monique Culturato ^{[1, 2]} ; de Jesus, Marcelo Bispo ^[1] ; van Gestel, Cornelis A. M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, BR-13083970 Campinas, SP - Brazil

^[2] Vrije Univ, Fac Sci, Dept Ecol Sci, De Boelelaan 1085, NL-1081 HV Amsterdam - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 715, MAY 1 2020.
• Citações Web of Science: 0

Resumo

We previously demonstrated that N-acetylcysteine (NAC) could reduce the toxicity of silver (Ag) materials (nanoparticles (NPs) and Ag nitrate) to the soil invertebrate Enchytraeus crypticus (Oligochaeta). It remains however, unclear whether the antitoxic mechanism of NAC was caused by NAC-Ag binding in the soil or inside the organisms. This study aimed at determining the bioavailability of Ag in the soil in a 21-day toxicity test as well as the Ag uptake and elimination kinetics in E. crypticus exposed to AgNPs in LUFA 2.2 standard soil amended with low (100 mg/kg dry soil) and high (600 mg/kg dry soil) NAC concentrations. The addition of NAC to the soil alleviated the toxicity of AgNPs by decreasing the internal Ag concentration of E. crypticus in a dose-dependent manner. Indeed, NAC reduced the binding of Ag to the soil, which probably was due to the formation of soluble but biologically unavailable Ag-cysteine complexes. The reduced Ag uptake in the enchytracids was explained from an increased elimination at high NAC levels. These findings reinforce the view that metal complexing-compounds like NAC play a key role in the modulation of AgNP toxicity and bioavailability in terrestrial environments. Further, it may inform on the potential of NAC as a remediation solution for Ag or other metal-contaminated soils. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 18/24658-9 - Uso da N-Acetilcisteína na remediação de solo contaminado com nanopartículas de prata: abordagem toxicocinética e toxicodinâmica em Enchytraeus crypticus
• Beneficiário: Monique Culturato Padilha Mendonça
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/03765-6 - Determinação in vitro e in vivo do perfil toxicológico de formulações utilizando nanotecnologia
• Beneficiário: Monique Culturato Padilha Mendonça
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado