A Protocol for Fish Lipid Analysis Using Nuclear Magnetic Resonance Spectroscopy

Autor(es)

Mor, Natalia Cristina ^[1] ; Correia, Banny S. B. ^[2] ; Val, Adalberto Luis ^[3] ; Tasic, Ljubica ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Ciencias Farmaceurt, R Candido Portinari 200, Cidade Univ, BR-13083871 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Dept Quim Organ, Inst Quim, Rua Jose de Castro S-N, Cidade Univ, BR-13083970 Campinas, SP - Brazil

^[3] Inst Nacl de Pesquisas da Amazonia, Lab Ecofisiol & Evolucao Mol, Coordenacao Biodivers, Ave Andre Araujo 2936, BR-69067375 Manaus, Amazonas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 31, n. 4, p. 662-672, APR 2020.
• Citações Web of Science: 0

Resumo

This article reports on an easy-to-follow methodology for fish lipids analysis, including the biological sample collection, lipid extraction, sample preparation, nuclear magnetic resonance (NMR) spectroscopy analysis and statistical data analysis, with details on parameters used for the NMR analysis and the care needed in each step execution. As an example of the biological matrix of lipids, for the entire procedure, we have used two Amazonian fish samples. Phenotype factor was taken into account when lipid contents of the Amazonian fish samples were evaluated, such as fish-eating habits in distinct Amazon seasonal periods, the flood and the drought. Results demonstrated the applicability of NMR as a powerful and useful tool for fish oil analysis. The omnivorous (T. elongatus) and the piscivorous (C. monoculus) Amazonian fish differ in composition and distribution of lipids, which show differences not only because of the different eating habits between the two species, but also by the availability and quantity of food along the year, i.e., during the flood and drought Amazon periods. (AU)

• Processo FAPESP: 13/14707-9 - Análise lipidômica de alguns peixes amazônicos
• Beneficiário: Banny Silva Barbosa Correia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/11258-1 - Análise de lipídeos isolados do fígado de duas espécies de peixes amazônicos aplicando ressonância magnética nuclear (RMN)
• Beneficiário: Natalia Cristina Mor
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica