Developing a screen-printed graphite-polyurethane composite electrode modified with gold nanoparticles for the voltammetric determination of dopamine

Autor(es)

Cervini, Priscila ^[1] ; Mattioli, Isabela A. ^[1] ; Cavalheiro, Eder T. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: RSC ADVANCES; v. 9, n. 72, p. 42306-42315, DEC 19 2019.
• Citações Web of Science: 0

Resumo

A screen-printed electrode (SPGPUE) was prepared with graphite-polyurethane composite ink containing gold nanoparticles (AuNPs), resulting in a screen-printed graphite-polyurethane composite electrode modified with gold nanoparticles (SPGPUE-AuNPs). Gold nanoparticles were prepared by the citrate method and extracted from the water medium since polyurethane is not compatible with humidity. After extraction to chloroform, they were characterized via transmission electron microscopy (TEM). The presence of gold on the SPGPUE-AuNP surface was confirmed via SEM and EDX analyses, while thermogravimetry revealed the presence of approximately 3.0% (m/m) gold in the composite. An electrochemical pretreatment in 0.10 mol L-1 phosphate buffer (pH 7.0) with successive cycling between -1.0 V and 1.0 V (vs. pseudo-Ag/AgCl) under a scan rate of 200 mV s(-1) and 150 cycles was required in order to provide a suitable electrochemical response for the voltammetric determination of dopamine. After the optimization of the parameters of differential pulse voltammetry (DPV), an analytical curve was obtained within a linear dynamic range of 0.40-60.0 mu mol L-1 and detection limit (LOD) of 1.55 x10(-8) mol L-1 for dopamine at the SPGPUE-AuNP. A non-modified SPGPUE was used for comparison and a linear range was obtained between 2.0 and 10 mu mol L-1 with an LOD of 2.94 x 10(-7) mol L-1. During the dopamine determination in cerebrospinal synthetic fluid (CSF), recoveries between 89.3 and 103% were achieved. There were no significant interferences from ascorbic acid and uric acid, but some from epinephrine due to the structural similarity. (AU)

• Processo FAPESP: 17/04211-7 - Desenvolvimento de tintas à base de nanopartículas metálicas e polímeros para construção de eletrodos compósitos convencional e impresso e suas aplicações
• Beneficiário: Éder Tadeu Gomes Cavalheiro
• Linha de fomento: Auxílio à Pesquisa - Regular