Aquaporin 1, 3, and 5 Patterns in Salivary Gland Mucoepidermoid Carcinoma: Expression in Surgical Specimens and an In Vitro Pilot Study

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Stamboni, Merin Barbara ^{[1, 2]} ; de Mello Gomes, Agatha Nagli ^[1] ; de Souza, Milena Monteiro ^[1] ; Oliveira, Katia Klug ^[1] ; Joca Arruda, Claudia Fabiana ^[3] ; de Paula, Fernanda ^[3] ; Bettim, Barbara Beltrame ^[1] ; Marques, Marcia Martins ^{[4, 5]} ; Kowalski, Luiz Paulo ^[6] ; Lopes Pinto, Clovis Antonio ^[7] ; Arana-Chavez, Victor Elias ^[8] ; Lourenco, Silvia Vanessa ^[3] ; Coutinho-Camillo, Claudia Malheiros ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Int Res Ctr, AC Camargo Canc Ctr, Rua Tagua 440, BR-01508010 Sao Paulo - Brazil

^[2] Natl Inst Sci & Technol Oncogen & Therapeut Innov, BR-05403010 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Dent Sch, Dept Gen Pathol, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, BR-05508000 Sao Paulo - Brazil

^[5] Univ Ibirapuera, Sch Dent, Postgrad Program, BR-04661100 Sao Paulo - Brazil

^[6] AC Camargo Canc Ctr, Dept Head & Neck Surg & Otorhinolaryngol, BR-01525001 Sao Paulo - Brazil

^[7] AC Camargo Canc Ctr, Dept Anat Pathol, BR-01525001 Sao Paulo - Brazil

^[8] Univ Sao Paulo, Dent Sch, Dept Biomat & Oral Biol, Lab Oral Biol, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 4 FEB 2020.
• Citações Web of Science: 0

Resumo

Salivary gland aquaporins (AQPs) are essential for the control of saliva production and maintenance of glandular structure. However, little is known of their role in salivary gland neoplasia. Salivary gland tumors comprise a heterogeneous group of lesions, featuring variable histological characteristics and diverse clinical behaviors. Mucoepidermoid carcinoma (MEC) is the most common salivary gland malignancy. The aim of this study was to evaluate the expression of AQP1, AQP3, and AQP5 in 24 MEC samples by immunohistochemistry. AQP1 expression was observed in vascular endothelium throughout the tumor stroma. AQP3 was expressed in epidermoid and mucosal cells and AQP5 was expressed in mucosal cells of MEC. These proteins were expressed in the human MEC cell line UH-HMC-3A. Cellular ultrastructural aspects were analyzed by electron microscopy to certificate the tumor cell phenotype. In summary, our results show that, despite the fact that these molecules are important for salivary gland physiology, they may not play a distinct role in tumorigenesis in MEC. Additionally, the in vitro model may offer new possibilities to further investigate mechanisms of these molecules in tumor biology and their real significance in prognosis and possible target therapies. (AU)

• Processo FAPESP: 11/02051-6 - Apoptose nas glândulas salivares humanas: avaliação de marcadores na morfogênese e no processo de tumorigênese da glândula utilizando como modelos o adenoma pleomórfico e o carcinoma mucoepidermóide
• Beneficiário: Cláudia Malheiros Coutinho Camillo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/50943-1 - INCT 2014: de Oncogenômica e Inovação Terapêutica
• Beneficiário: Dirce Maria Carraro
• Modalidade de apoio: Auxílio à Pesquisa - Temático