First isolation and genotyping of Toxoplasma gondii in a free-living giant anteater (Myrmecophaga tridactyla) revealed a unique non-archetypal genotype

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Jesus Pena, Hilda Fatima ^[1] ; Ferrari, Vinicius Matheus ^{[2, 3]} ; Nogueira Aires, Luiz Paulo ^{[2, 4]} ; Soares, Herbert Sousa ^{[1, 5]} ; Oliveira, Solange ^{[1, 6]} ; Alves, Bruna Farias ^[1] ; Gennari, Solange Maria ^{[1, 5]} ; Dubey, Jitender P. ^[7] ; de Mattos, Luiz Carlos ^[2] ; Brandao de Mattos, Cinara Cassia ^[2] ; Castiglioni, Lilian ^{[8, 3, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Doencas Parasitarias, Dept Med Vet Prevent & Saude Anim VPS, Fac Med Vet & Zootecnia, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

^[2] Fac Med Sao Jose Rio Preto FAMERP, Dept Biol Mol, FAMERP Toxoplasma Res Grp, Sao Paulo - Brazil

^[3] Univ Estadual Paulista, UNESP, IBILCE, Campus Sao Jose do Rio Preto, Sao Paulo - Brazil

^[4] Ctr Univ Rio Preto UNIRP, Sao Paulo, SP - Brazil

^[5] Univ Santo Amaro, Dept Mestrado Med & Bem Estar Anim, Campus 1, Sao Paulo - Brazil

^[6] Ctr Ensino Tecn, Fac Metodo Sao Paulo, Sao Paulo - Brazil

^[7] USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Beltsville, MD 20705 - USA

^[8] Fac Med Sao Jose Rio Preto FAMERP, Dept Epidemiol & Saude Colet, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Tropica; v. 204, APR 2020.
• Citações Web of Science: 0

Resumo

The protozoan parasite Toxoplasma gondii can infect virtually all warm-blooded animals worldwide but little is known of its infection in the endangered giant anteaters (Myrmecophaga tridactyla). The present study found antibodies to T. gondii in 13 of 23 free-living M. tridactyla from the northwest region of Sao Paulo state, Brazil, by the Modified Agglutination Test (MAT, cut-off titer 1:25). Unfrozen tissues of seven M. tridactyla were bioassayed in mice and viable T. gondii (strain designated TgMytrBrSP1) isolated from one seropositive giant anteater. To our knowledge, this is a new host record for T. gondii. Genotyping using PCR-RFLP revealed the Brazilian clonal Type BrIII genotype, and a unique non-archetypal genotype was revealed by microsatellite analysis. (AU)

• Processo FAPESP: 14/05302-8 - Isolamento e caracterização genética de Toxoplasma gondii em Myrmecophaga tridactyla (Linnaeus, 1758)
• Beneficiário: Lilian Castiglioni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/25872-3 - Caracterização genética de Toxoplasma gondii em Myrmecophaga tridactyla (Linnaeus, 1758)
• Beneficiário: Luiz Paulo Nogueira Aires
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica