Self-Assembly, Nematic Phase Formation, and Organocatalytic Behavior of a Proline-Functionalized Lipopeptide

Autor(es)

Pelin, Juliane N. B. D. ^{[1, 2]} ; Edwards-Gayle, Charlotte J. C. ^[2] ; Castelletto, Valeria ^[2] ; Aguilar, Andrea M. ^[3] ; Alves, Wendel A. ^[1] ; Seitsonen, Jani ^[4] ; Ruokolainen, Janne ^[4] ; Hamley, Ian W. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil

^[2] Univ Reading, Dept Chem, Reading RG6 6AD, Berks - England

^[3] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, BR-09972270 Diadema - Brazil

^[4] Aalto Univ, Nanomicroscopy Ctr, FIN-02150 Espoo - Finland

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 12, n. 12, p. 13671-13679, MAR 25 2020.
• Citações Web of Science: 4

Resumo

The self-assembly of the amphiphilic lipopeptide PAEPKI-C-16 (P = proline, A = alanine, E = glutamic acid, K = lysine, I = isoleucine, and C(16 )hexadecyl) was investigated using a combination of microscopy, spectroscopy, and scattering methods and compared to that of C-16-IKPEAP with the same (reversed) peptide sequence and the alkyl chain positioned at the N-terminus and lacking a free N-terminal proline residue. The catalytic activity of these peptides was then compared using a model aldol reaction system. For PAEPKI-C-16, the cryo-TEM images showed the formation of micrometer-length fibers, which by small-angle X-ray scattering (SAXS) were found to have radii of 2.5-2.6 nm. Spectroscopic analysis shows that these fibers are built from beta-sheets. This behavior is in complete contrast to that of C-16-IKPEAP, which forms spherical micelles with peptides in a disordered conformation {[}Hutchinson et al. J. Phys. Chem. B 2019, 123, 613]. In PAEPKI-C-16, spontaneous alignment of fibers was observed upon increasing pH, which was accompanied by observed birefringence and anisotropy of SAXS patterns. This shows the ability to form a nematic phase, and unprecedented nematic hydrogel formation was also observed for these lipopeptides at sufficiently high concentrations. SAXS shows retention of an ultrafine (1.7 nm core radius) fibrillar network within the hydrogel. PAEPKI-C-16 with free N-terminal proline shows enhanced anti:syn diastereoselectivity and better conversion compared to C-16-IKPEAP. The cytotoxicity of PAEPKIC(16) was also lower than that of C-16-IKPEAP for both fibroblast and cancer cell lines. These results highlight the sensitivity of lipopeptide properties to the presence of a free proline residue. The spontaneous nematic phase formation by PAEPKI-C-16 points to the high anisotropy of its ultrafine fibrillar structure, and the formation of such a phase at low concentrations in aqueous solution may be valuable for future applications. (AU)

• Processo FAPESP: 15/20446-9 - Estudo da auto-organização de estruturas hierárquicas de peptídeos em solução em presença de nanopartículas metálicas
• Beneficiário: Juliane Nogueira Batista Dias Pelin
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/12535-0 - Estudo da auto-organização de estruturas hierárquicas de peptídeos em solução em presença de nanopartículas metálicas
• Beneficiário: Juliane Nogueira Batista Dias Pelin
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 17/02317-2 - Interfaces em materiais: propriedades eletrônicas, magnéticas, estruturais e de transporte
• Beneficiário: Adalberto Fazzio
• Modalidade de apoio: Auxílio à Pesquisa - Temático