| Texto completo | |
| Autor(es): |
Frigori, Rafael B.
[1]
;
Barroso da Silva, Fernando L.
[2]
;
Carvalho, Patricia P. D.
[3]
;
Alves, Nelson A.
[3]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Tecnol Fed Parana, BR-85902490 Toledo, Parana - Brazil
[2] Univ Sao Paulo, Dept Ciencias Biomol, FCFRP, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Dept Fis, FFCLRP, BR-14040901 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Physical Chemistry B; v. 124, n. 14, p. 2798-2805, APR 9 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
We investigate the prevalence, and so the role in the amyloidogenesis, of biased conformations in large ensembles of monomeric forms for A eta 42 and A beta 40 that can trigger the formation and growth of fibrils described by a dock-lock mechanism. We model such biased conformations as the structural monomeric units that constitute the Protein Data Bank fibrils 2beg, 2mxu, and 2lmn. These units were employed as templates to search for similar structures in statistical conformational ensembles of A beta peptides generated by molecular dynamics with an accurate force field in explicit solvation, whose high quality is revealed by comparison with residual dipolar coupling (RDC) experiments. The conformational ensembles generated by these intrinsically disordered peptides do not contain conformations highly similar to the amyloidogenic templates. This is a consequence of the low thermodynamic stability exhibited by the template-like conformations. A further constant-pH Monte Carlo study has revealed that this stability can be increased by suitable pH conditions, which helps to trigger the fibril elongation. Moreover, our analyses on the free energy landscapes, hydrogen bond prevalences, and principal component analysis distributions emphasize the relevance of many-body long-range cooperative interactions, likely acting over the infrequent preexisting structurally biased conformations, to explain the fibrils' emergence. (AU) | |
| Processo FAPESP: | 15/16116-3 - Mecanismos moleculares de origem eletrostática responsáveis pela complexação de proteínas |
| Beneficiário: | Fernando Luis Barroso da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/04176-4 - Análise sistemática in silico do comportamento crítico do peptídeo amiloide beta e de alguns dos seus mutantes |
| Beneficiário: | Nelson Augusto Alves |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |