Co-occurrence of mcr-1, mcr-3, mcr-7 and clinically relevant antimicrobial resistance genes in environmental and fecal samples

Autor(es)

Rodrigues dos Santos, Lucas David ^[1] ; Rueda Furlan, Joao Pedro ^[1] ; Ramos, Micaela Santana ^[1] ; Lage Gallo, Inara Fernanda ^[1] ; Pereira de Freitas, Lucas Victor ^[2] ; Stehling, Eliana Guedes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe S-N Monte Alegre, Ribeirao Preto 14040903 - Brazil

^[2] Univ Fed Minas Gerais UFMG, Montes Claros, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Microbiology; v. 202, n. 7 MAY 2020.
• Citações Web of Science: 0

Resumo

Multidrug-resistant bacteria harboring different antimicrobial resistance genes (ARGs) have been detected worldwide. The association of plasmid-mediated colistin resistance genes (mcr-like) and other ARGs in bacteria isolated from animals is a huge concern worldwide. Therefore, this study aimed to investigate the presence of mcr-like genes and clinically relevant ARGs as well as plasmids in samples from a zoo. Fecal and environmental (soil and water) samples were collected from a zoo and the DNA of cultivable aerobic bacteria was extracted. ARGs were screened by PCR and the plasmids were detected using the PCR-based replicon typing method. A total of 74 amplicons from 27 ARGs {[}mcr-1, mcr-3, mcr-7.1, bla(CTX-M-Gp1), bla(CTX-M-Gp2), bla(CTX-M-Gp9), bla(VEB), bla(PER), bla(CMY), tetA, tetB, tetC, aadA, aac(6 `)-Ib, aph(3 `)-Ia, ant(2 `')-Ia, qnrA, qnrB, qnrS, oqxA, oqxB, sul1, sul2, sul3, cmlA, mefAE, ermB] and 21 amplicons from eight plasmid families (IncY, ColE-like, IncF(repB), IncFIA, IncFIB, IncHI1, IncFIC, IncP) were detected. These findings reinforce that the zoo acts as a reservoir of clinically relevant ARGs, including mcr-like, and call attention to the monitoring studies in the zoo. Therefore, to the best of our knowledge, this is the first report of the world of mcr-1, mcr-3 and mcr-7.1 in environmental samples from the zoo. (AU)

• Processo FAPESP: 18/19539-0 - Caracterização molecular de isolados de Escherichia coli provenientes do meio ambiente
• Beneficiário: Eliana Guedes Stehling
• Modalidade de apoio: Auxílio à Pesquisa - Regular