Applying a pan-flavivirus RT-qPCR assay in Brazilian public health surveillance

Autor(es)

Cunha, Mariana Sequetin ^[1] ; Luchs, Adriana ^[2] ; Pereira dos Santos, Fabiana Cristina ^[3] ; Caleiro, Giovana Santos ^{[1, 4]} ; Nogueira, Mauricio Lacerda ^[5] ; Maiorka, Paulo Cesar ^[6]

Afiliação do(s) autor(es)

^[1] Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Lab, Sao Paulo - Brazil

^[2] Adolfo Lutz Inst, Virol Ctr, Enter Dis Lab, Sao Paulo - Brazil

^[3] Adolfo Lutz Inst, Virol Ctr, Resp Dis Lab, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sao Paulo Inst Trop Med, Virol Lab, Sao Paulo - Brazil

^[5] Fac Med Sao Jose do Rio Preto FAMERP, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 165, n. 8 MAY 2020.
• Citações Web of Science: 0

Resumo

The aim of this study was to improve flavivirus field monitoring in Brazil using a reliable probe-based RT-qPCR assay. Standard flavivirus strains were employed to evaluate the performance of the assay, and its applicability was evaluated using 235 stored pools of Culicidae samples collected between 1993 and 1997 and in 2016. Flavivirus species were identified by sequencing. Sixteen (6.8%) samples tested positive: Ilheus virus, Iguape virus, and Saint Louis encephalitis virus were identified in historical specimens from 1993-1994, while insect-specific flaviviruses were detected in the samples from 2016. This approach was demonstrated to be accurate for flavivirus detection and characterization, and it can be successfully applied for vector surveillance and for monitoring and discovery of insect specific flaviviruses. (AU)

• Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
• Beneficiário: Maurício Lacerda Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/23645-4 - Estudo de alterações patológicas em situações ligadas à perícia em medicina veterinária
• Beneficiário: Paulo César Maiorka
• Modalidade de apoio: Auxílio à Pesquisa - Regular