Photophysics of J-Aggregating Porphyrin-Lipid Photosensitizers in Liposomes: Impact of Lipid Saturation

Autor(es)

Charron, Danielle M. ^{[1, 2]} ; Yousefalizadeh, Goonay ^[3] ; Buzza, Hilde H. ^[4] ; Rajora, Maneesha A. ^{[1, 2]} ; Chen, Juan ^[1] ; Stamplecoskie, Kevin G. ^[3] ; Zheng, Gang ^{[1, 2, 5]}

Afiliação do(s) autor(es)

^[1] Univ Hlth Network, Princess Margaret Canc Ctr, Toronto, ON M5G 1L7 - Canada

^[2] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3G9 - Canada

^[3] Queens Univ, Dept Chem, Kingston, ON K7L 3N6 - Canada

^[4] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13563120 Sao Carlos - Brazil

^[5] Univ Toronto, Dept Med Biophys, Toronto, ON M5S 3G9 - Canada

• Tipo de documento: Artigo Científico
• Fonte: Langmuir; v. 36, n. 19, p. 5385-5393, MAY 19 2020.
• Citações Web of Science: 0

Resumo

Porphyrin aggregates have attractive photophysical properties for phototherapy and optical imaging, including quenched photosensitization, efficient photothermal conversion, and unique absorption spectra. Although hydrophobic porphyrin photosensitizers have long been encapsulated into liposomes for drug delivery, little is known about the membrane properties of liposomes with large amphiphilic porphyrin compositions. In this paper, a porphyrin-lipid conjugate was incorporated into liposomes formed of saturated or unsaturated lipids to study the membrane composition-dependent formation of highly ordered porphyrin J-aggregates and disordered aggregates. Porphyrin-lipid readily phase-separates in saturated membranes, forming J-aggregates that are destabilized during the ripple phase below the main thermal transition. Porphyrin-lipid J-aggregates are photostable with a photothermal efficiency of 54 +/- 6%, comparable to gold. Even at high porphyrin-lipid compositions, porphyrin J-aggregates coexist with a minority population of disordered aggregates, which are photodynamically active despite being fluorescently quenched. For photothermal applications, liposome formulations that encourage porphyrin-lipid phase separation should be explored for maximum J-aggregation. (AU)

• Processo FAPESP: 17/26710-5 - Estudo de porfissomos e agregados-J e o entendimento da distribuição da luz do calor para aplicação em terapia fototérmica para tratamento de tumores
• Beneficiário: Hilde Harb Buzzá
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/14033-6 - Estudos básicos da combinação dos efeitos fototérmico e fotodinâmico na aplicação de uma nova modalidade para o tratamento do Câncer
• Beneficiário: Hilde Harb Buzzá
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado