In vitro effects of photobiomodulation applied to gingival fibroblasts cultured on titanium and zirconia surfaces and exposed to LPS fromEscherichia coli

Autor(es)

Pansani, Taisa Nogueira ^[1] ; Basso, Fernanda Goncalves ^[2] ; de Souza Costa, Carlos Alberto ^{[3, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] Univ Ribeirao Preto, UNAERP, Dept Dent, Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Humaita, 1680 Ctr, BR-14801903 Araraquara, SP, Brazil.Pansani, Taisa Nogueira, Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[4] Univ Estadual Paulista, UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Humaita, 1680 Ctr, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 35, n. 9 JUN 2020.
• Citações Web of Science: 0

Resumo

Photobiomodulation (PBM) therapy is used to stimulate cell proliferation and metabolism, as well as reduce inflammatory cytokine synthesis, which plays a main role in the long-term stability of implants. This study assessed the response of gingival fibroblasts cultured on titanium (Ti) and zirconia (ZrO2), submitted to PBM and exposed to lipopolysaccharide (LPS). Cells seeded on Ti and ZrO(2)were irradiated (InGaAsP; 780 nm, 25 mW) 3 times, using 0.5, 1.5, and 3.0 J/cm(2)doses, and exposed toEscherichia coliLPS (1 mu g/mL). After 24 h, cell viability (alamarBlue,n = 8), interleukin 6 (IL-6) and 8 (IL-8) synthesis (ELISA,n = 6), andIL-6and vascular endothelial growth factor (VEGF) gene expression (qPCR,n = 5) were assessed and statistically analyzed (one-way ANOVA,alpha = 0.05). Cell morphology was evaluated by fluorescence microscopy. Increased cell viability occurred in all groups cultured on Ti compared with that of the control, except for cells exposed to LPS. Fibroblasts cultured on ZrO(2)and LPS-exposed exhibited reduced viability. PBM at 3.0 J/cm(2)and 1.5 J/cm(2)downregulated the IL-6 synthesis by fibroblasts seeded on Ti and ZrO2, as well as IL-8 synthesis by cells seeded on ZrO2. Fibroblasts seeded on both surfaces and LPS-exposed showed increasedIL-6gene expression; however, this activity was downregulated when fibroblasts were irradiated at 3.0 J/cm(2). EnhancedVEGFgene expression by cells seeded on Ti and laser-irradiated (3.0 J/cm(2)). Distinct patterns of cytoskeleton occurred in laser-irradiated cells exposed to LPS. Specific parameters of PBM can biomodulate the inflammatory response of fibroblasts seeded on Ti or ZrO(2)and exposed to LPS. (AU)

• Processo FAPESP: 15/19364-8 - Efeito da laserterapia de baixa potência e do fator de crescimento epidérmico sobre a indução do selamento biológico in vitro
• Beneficiário: Taisa Nogueira Pansani
• Modalidade de apoio: Bolsas no Brasil - Doutorado