Turning the Screw: Engineering Extreme pH Resistance in Escherichia coli through Combinatorial Synthetic Operons

Autor(es)

de Siqueira, V, Guilherme M. ; Silva-Rocha, Rafael ^[1] ; Guazzaroni, Maria-Eugenia ^[2]

Afiliação do(s) autor(es)

^[1] Fac Med Ribeirao Preto FMRP USP, Dept Biol Celular & Mol & Bioagentes Patog, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Fac Filosofia Crencias & Letras Ribeirao Preto FF, Dept Biol, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS SYNTHETIC BIOLOGY; v. 9, n. 6, p. 1254-1262, JUN 19 2020.
• Citações Web of Science: 0

Resumo

Adoption of microorganisms as platforms for sustainable biobased production requires host cells to be able to withstand harsh conditions, usually very distant from those in which these organisms are naturally adapted to thrive. However, novel survival mechanisms unearthed by the study of microbiomes from extreme habitats may be exploited to enhance microbial robustness under the strict conditions needed for different industrial appplications. In this work, synthetic biology approaches were used to engineer enhanced acidic resistance in Escherichia coli through the characterization of a collection of unique operons composed of combinatorial assemblies of three novel genes from an extreme environment and three synthetic ribosome binding sites. The results here presented illustrate the efficacy of combining different metagenomic genes for resistance in synthetic operons, as expression of these gene clusters increased hundred-fold the survival percentage of cells exposed to an acidic shock in minimal media at pH 1.9 under aerobic conditions. (AU)

• Processo FAPESP: 15/04309-1 - Novas abordagens para melhorar a prospecção funcional de biocatalizadores em bibliotecas metagenômicas
• Beneficiário: María Eugenia Guazzaroni
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/07261-8 - Expandindo a resistência a acidez em bactérias utilizando circuitos sintéticos
• Beneficiário: Guilherme Marcelino Viana de Siqueira
• Modalidade de apoio: Bolsas no Brasil - Mestrado